FastQCFastQC Report
Wed 25 May 2016
SRR1780799_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780799_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences281759
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCCGT3584312.721155313583596No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCCG68212.4208632199858746No Hit
CATATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCCGT48561.7234587005206576No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCC20990.7449628938206055No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG20790.737864628991443No Hit
CTAATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCCGT13090.4645814330686864No Hit
CTTAAACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCCGT11100.39395369801851937No Hit
TCTTTATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCC8980.3187120908293968No Hit
ATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCCGTCTT7230.25660227357422477RNA PCR Primer, Index 19 (95% over 23bp)
CAAATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCCGT6890.2445352233646485No Hit
TCATATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCCG6390.22678956129174224No Hit
CATAAACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCCGT5820.20655950652862906No Hit
CTTATACACATCTCCGCGCCCACGAGACTTATGAAAATCTCGTATGCCGT3110.11037801809347705No Hit
CTTATACACATCTCCGAGCCCACGAGACTTAAGAAAATCTCGTATGCCGT2850.10115027381556578No Hit
CTTATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGACGT2850.10115027381556578No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAACCG207.9106896E-570.013510
CACGAAT207.917649E-570.0010739
CCTTGAA207.917649E-570.0010756
AAGCTTG353.6998244E-970.0010654
GTATCCC150.002224587869.9886442
CGTCTAC1100.066.819248
AGGGGGG53900.065.5854170
CTACTGC1150.060.88129851
AACTCGT1050.060.00090837
GCCGTAT650.059.2316746
GATCTCG308.362995E-658.3342236
TCGTATC308.371799E-658.32386840
AGCCGTC1450.057.9319245
AAGCCGT1450.057.9319244
ATACGAG553.6379788E-1157.28376812
TGCCGTA800.056.87586645
AAACTCG1300.056.5393236
AATCACG1300.056.5393236
CGTCTTA1300.056.5393248
CACGTAT1250.056.00085439
Produced by FastQC (version 0.11.5)