FastQCFastQC Report
Wed 25 May 2016
SRR1780798_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780798_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences962063
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG573375.959796811643312No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCCGT1500.044.33843652
GGACCTA801.4242687E-939.375441
GTCGCCG1950.037.69468751
ACGACGT400.002417255235.00584870
GCCGTAT1400.035.0040354
GATCATA1102.3646862E-1135.0003851
TGGTCGC1650.033.93977449
TCGGTGG1700.032.9449745
CCGCATT1251.05501385E-1030.80354752
CGGTGGT2050.030.73524546
CGCATTC1401.2732926E-1130.00345453
GGGTCTA853.95777E-628.8238491
CACGACG500.007203913828.0046869
CGCCGTA2100.026.66973753
CTCGGTG2000.026.25165744
CGTGCGA1101.017439E-625.4521810
CCGTGCG1101.017439E-625.452189
CCAACGA1551.2860255E-924.84544668
GTAGATC2400.024.79194538
AACGAGC1701.4006218E-1024.7100170
Produced by FastQC (version 0.11.5)