FastQCFastQC Report
Wed 25 May 2016
SRR1780798_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780798_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences962063
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTTAATGCATCTCGTATGCCGT329213.421917275687767No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTTAATGCATCTCGTATGCCG52260.543207669352215No Hit
CTAATACACATCTCCGAGCCCACGAGACTTTAATGCATCTCGTATGCCGT31110.3233675965087526No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTTTAATGCATCTCGTATGCC17870.18574667147577653No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG45950.061.6235770
CGTCTTC60100.054.85201348
GCCGTCT61350.054.47879446
CCGTCTT60700.054.257847
CGTATGC63850.054.0477141
TCGTATG64700.054.04088640
TATGCCG63500.054.00929343
CTCGTAT64000.053.87195239
TCTCGTA64450.053.49580438
CTTGAAA63550.053.25921257
ATCTCGT64500.053.0744637
ATGCCGT64800.053.03653744
GTATGCC64900.053.0059942
TCTTCTG60750.053.00329250
TGCCGTC64800.052.9285245
GTCTTCT61300.052.75062649
CTGCTTG60850.052.63680354
CATCTCG65500.052.58479736
ATCGTAT1400.052.50464239
ACGTATT200.00695212352.4991847