FastQCFastQC Report
Wed 25 May 2016
SRR1780797_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780797_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences224739
Sequences flagged as poor quality0
Sequence length76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4016117.870062605956242No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCCGAT150.002222765269.9984453
ACCCGAT150.002222765269.998447
CGACTAA252.3845237E-456.01121537
TCCGATA200.006945407552.49883354
CAGTTGT200.006945407552.4988331
TCTAGCC200.006945407552.4988333
GATCCCC305.869898E-446.665636
CTCGGTG551.3065437E-744.5444644
ACTAATT404.583557E-543.74902718
TCGGTGG659.964424E-943.07596645
TGGTCGC602.5878217E-740.83242849
CACCTGG350.001253751539.9991077
TATCTCA350.001253751539.99910722
TCTCGGT701.916851E-839.99910743
TCGCCGT459.179488E-538.8880252
TCGCCCC400.00241293435.0070138
CCGACTA400.00241293435.0070136
GATCGGA400.002415570734.9992211
ATTGATC400.002415570734.999223
ATGCAGG400.002415570734.9992259
Produced by FastQC (version 0.11.5)