FastQCFastQC Report
Wed 25 May 2016
SRR1780797_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780797_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences224739
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCCGT219689.77489443309795No Hit
TCTTATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCCG35301.5707109135486053No Hit
CTAATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCCGT17860.7946996293478212No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCC12720.5659898815959846No Hit
CTTATACACATCTCCGCGCCCACGAGACCTCATGGGATCTCGTATGCCGT11620.5170442157346967No Hit
CATATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCCGT6480.2883344679828601No Hit
TCTTTATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCC5520.2456182505039179No Hit
ATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCCGTCTT5090.22648494475814165RNA PCR Primer, Index 34 (95% over 23bp)
TCATATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCCG4550.20245707242623665No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4010.17842920009433164No Hit
TCTTATACACATCTCCGCGCCCACGAGACCTCATGGGATCTCGTATGCCG3030.13482306141791145No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCATGGGATATCGTATGCCGT2870.12770369183808775No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATTTGG150.002221223770.0106864
CGCGAAC150.002221223770.0106823
CAACCGG150.002223185569.9951144
ATTGTCG150.002223185569.9951147
TCGGATC150.002223185569.9951133
AACATTG150.002223185569.9951142
ATCGGAT150.002223185569.9951132
CTCTCGA150.002223185569.9951137
AGGGGGG27350.063.2131370
CTCGAAA308.361725E-658.32925439
AACTCGA308.361725E-658.32925437
GATCTCC553.6379788E-1157.26872336
CGTATGA1250.055.99608241
CCGACTT750.055.99608247
GCCGACT700.054.99615546
CGACTTC700.054.99615548
ATCAGCA452.687375E-854.4527552
TGGGAAC900.054.44063633
GTCTATC200.00694060552.50801553
GGTCTAT200.00694060552.50801552
Produced by FastQC (version 0.11.5)