FastQCFastQC Report
Wed 25 May 2016
SRR1780796_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780796_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28371
Sequences flagged as poor quality0
Sequence length76
%GC88

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1811963.86450953438371No Hit
CTTATACACATCTGACGCTGCCGACGAGCCACGTCGTGTAGATCTCGGTG830.29255225406224666No Hit
GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG470.16566211977018785No Hit
GGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG460.16213739381763068No Hit
GGGGGGGGGGGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG440.1550879419125163No Hit
GGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG390.13746431214973037No Hit
GGGGGGGGGGGGGGGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGG290.10221705262415848No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGGCG207.796204E-570.0000120
AGATCTC207.796204E-570.0000140
ATCTCGG252.770128E-670.042
TATACGC150.002202527670.03
TGCCGGC150.002202527670.019
TCGGTGG353.5433914E-970.045
TTATACG150.002202527670.02
TCTCGGT309.886935E-870.043
GATCTCG252.770128E-670.041
CTCGGTG309.886935E-870.044
TGGCGCT150.002202527670.013
GTCGCCG352.8729846E-760.051
CGGTGGT352.8729846E-760.046
GGTGGTC352.8729846E-760.047
GTGGTCG352.8729846E-760.048
CGCCGTA352.8729846E-760.053
GGTCGCC352.8729846E-760.050
TGGTCGC308.1720755E-658.33333249
TCGCCGT308.1720755E-658.33333252
CGTATCA308.1720755E-658.33333256
Produced by FastQC (version 0.11.5)