FastQCFastQC Report
Wed 25 May 2016
SRR1780796_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780796_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28371
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCCGT820928.93447534454196No Hit
TCTTATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCCG18906.661732050333087No Hit
CTAATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCCGT10783.7996545768566494No Hit
TCATATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCCG3941.3887420253075324No Hit
ATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCCGTCTT3691.3006238764936027TruSeq Adapter, Index 10 (95% over 21bp)
CTCTTATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCC3601.268901342920588No Hit
TCTTTATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCC3411.2019315498220013No Hit
CATATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCCGT3301.1631595643438721No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2610.9199534736174263No Hit
CTTATACACATCTCCGCGCCCACGAGACACCGGCTAATCTCGTATGCCGT2180.7683902576574672No Hit
CTATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCCGTC1390.48993690740544926No Hit
CAAATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCCGT1330.46878855169010614No Hit
CTTATACACATCTCCGAGCCCACGAGACACAGGCTAATCTCGTATGCCGT1010.3559973212082761No Hit
CTTTACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCCGTC960.3383736914454901No Hit
TCTTATACACATCTCCGCGCCCACGAGACACCGGCTAATCTCGTATGCCG940.3313242395403757No Hit
CTTATACACATCTCCGAGCCCACGAGACACCGGCAAATCTCGTATGCCGT850.299601705967361No Hit
CTTATACACATCTCCGAGCCCACGAGACACCGGCTAATATCGTATGCCGT850.299601705967361No Hit
TCTTAATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCC840.2960769800148038No Hit
CTTATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCCGA760.26787917239434633No Hit
CTTAAACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCCGT680.23968136477388885No Hit
CTTAATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCCG650.2291071869162173No Hit
CTTATACACATCTCCGAGCCCACGAGACACCGGCTATCTCGTATGCCGTC630.22205773501110287No Hit
TATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCCGTCT600.2114835571534313No Hit
CTTATACACATCTCCGAGCCCACGAGACAACGGCTAATCTCGTATGCCGT600.2114835571534313No Hit
CTTTATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCCG590.2079588312008741No Hit
CTTATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGACGT590.2079588312008741No Hit
TTATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCCGTC580.20443410524831696No Hit
CTTATACACATCTCCGAGCCCACGAGACACCGGATAATCTCGTATGCCGT570.20090937929575975No Hit
CTTATACACATCTCCGAGCCCACGAGACACCGGCTAAACTCGTATGCCGT540.1903352014380882No Hit
TCATTATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCC530.186810475485531No Hit
CTAATACACATCTCCGCGCCCACGAGACACCGGCTAATCTCGTATGCCGT520.18328574953297383No Hit
TCTATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCCGT460.16213739381763068No Hit
CCTTATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCCG460.16213739381763068No Hit
ATTATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCCGT410.14451376405484473No Hit
CTTATACACATCACCGAGCCCACGAGACACCGGCTAATCTCGTATGCCGT390.13746431214973037No Hit
TCTTATACACATCTCCGAGCCCACGAGCCACCGGCTAATCTCGTATGCCG390.13746431214973037No Hit
CTAATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGACGT390.13746431214973037No Hit
CTAATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCCGA380.13393958619717317No Hit
CTTATACACATCTCCGAGCCAACGAGACACCGGCTAATCTCGTATGCCGT380.13393958619717317No Hit
CTTATACACATCTCCGAGCCCACGAGACCCCGGCTAATCTCGTATGCCGT380.13393958619717317No Hit
CTAAAACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCCGT370.130414860244616No Hit
TCTTATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCAG350.1233654083395016No Hit
CTTATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCAGT330.11631595643438722No Hit
CTAATACACATCTCCGAGCCCACGAGACACCGGCTAATATCGTATGCCGT330.11631595643438722No Hit
CTTATACACATCTCCGAGCCCACGAGCCACCGGCTAATCTCGTATGCCGT320.11279123048183004No Hit
CTAATACACATCTCCGAGCCCACGAGACACCGGCTAAACTCGTATGCCGT310.10926650452927286No Hit
CTTATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTAAGCCGT300.10574177857671566No Hit
TCTTATACACATCTCCGAGCCCACGAGACACCGGCTAATATCGTATGCCG300.10574177857671566No Hit
TCTTATACACATCTCCGAGCCCACGAGACACCGGCTACTCTCGTATGCCG300.10574177857671566No Hit
TCCTTATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCC300.10574177857671566No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGCTT207.796204E-570.0000153
ATCTCGA401.2732926E-1070.0000137
CGGGGGG207.796204E-570.0000170
TCAAATA207.796204E-570.000011
TCTCGAA401.2732926E-1070.0000138
CTACGAG207.796204E-570.0000112
GCCGACA207.796204E-570.0000146
CCGACAT207.796204E-570.0000147
CGACATC207.796204E-570.0000148
CTACAGC150.002202527670.051
CTCGAAA150.002202527670.039
CCTGCTA150.002202527670.030
ATCTACG150.002202527670.010
TCGAAAG150.002202527670.040
GAGCCCC150.002202527670.016
TCTACGA150.002202527670.011
TCAGCAT150.002202527670.053
CCCCGAG150.002202527670.020
TCAGAGC150.002202527670.013
CCCGAGA150.002202527670.021
Produced by FastQC (version 0.11.5)