FastQCFastQC Report
Wed 25 May 2016
SRR1780795_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780795_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47769
Sequences flagged as poor quality0
Sequence length76
%GC92

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3407671.33496619146308No Hit
CTTATACACATCTGACGCTGCCGACGAGCCACGTCGTGTAGATCTCGGTG1840.38518704599217063No Hit
GGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG740.1549121815403295No Hit
GGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG660.1381649186711047No Hit
GGGGGGGGGGGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG610.12769787937783916No Hit
GGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG510.10676380079130818No Hit
GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG500.10467039293265508No Hit
GGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG480.10048357721534887No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGCGT150.002205655770.049265
ACGCGGC150.002205655770.049266
CGCGTCT207.8237834E-570.049267
GGCGCTG207.8645695E-569.97591414
TGCCGGC150.002214853469.97591419
TCTGGCG150.002214853469.97591411
GTCTGGC150.002214853469.97591410
CTGGCGC150.002214853469.97591412
GCGTCTG207.8645695E-569.9759148
CTGCCGG150.002214853469.97591418
CGTCTGG207.8645695E-569.9759149
CGCCGTA453.6925485E-1062.26601453
CCGTATC401.0326403E-861.29310655
CATTAAA401.0326403E-861.29310661
TCGCCGT401.0326403E-861.29310652
GCCGTAT401.0326403E-861.29310654
GTATCAT352.9013427E-760.04222557
CGTATCA352.9013427E-760.04222556
TATCATT352.9013427E-760.04222558
ATCATTA352.9013427E-760.04222559