FastQCFastQC Report
Wed 25 May 2016
SRR1780795_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780795_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47769
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCGT1775737.17264334610312No Hit
TCTTATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCG30426.368146706022734No Hit
CTAATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCGT14863.110804077958509No Hit
CTTATACACATCTCCGCGCCCACGAGACCGGTTCCCATCTCGTATGCCGT8171.7103142205195838No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCC7891.651698800477297No Hit
CATATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCGT4891.0236764428813665No Hit
TCTTTATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCC4720.9880885092842638No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4520.9462203521112019No Hit
TCATATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCG4360.9127258263727521No Hit
ATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCGTCTT4090.8562038141891184RNA PCR Primer, Index 14 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACCGGATCCCATCTCGTATGCCGT2170.45426950532772303No Hit
TCTTATACACATCTCCGCGCCCACGAGACCGGTTCCCATCTCGTATGCCG2050.4291486110238858No Hit
CTATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCGTC1960.41030794029600787No Hit
CTTATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGACGT1920.4019343088613955No Hit
CTTATACACATCTCCGAGCCCACGAGACCGGTTACCATCTCGTATGCCGT1860.38937386170947685No Hit
CAAATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCGT1780.372626598840252No Hit
CTTATACACATCTCCGAGCCCACGAGACCGGTTCACATCTCGTATGCCGT1520.3181979945152714No Hit
CTTATACACATCTCCGAGCCCACGAGACCGGTTCCCATATCGTATGCCGT1330.2784232452008625No Hit
CTTATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCGA1200.25120894303837216No Hit
CTTATACACATCTCCGAGCCCACGAGCCCGGTTCCCATCTCGTATGCCGT1170.24492871946241285No Hit
CTTATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTAAGCCGT1110.23236827231049423No Hit
CTTTACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCGTC1030.2156210094412694No Hit
CTTATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCAGT990.20724737800665705No Hit
CTTATACACATCTCCGAGCCCACGAGACCGGTTCCAATCTCGTATGCCGT980.20515397014800393No Hit
CTTAAACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCGT940.19678033871339154No Hit
TCTTAATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCC930.19468693085473843No Hit
CTAATACACATCTCCGCGCCCACGAGACCGGTTCCCATCTCGTATGCCGT920.19259352299608531No Hit
CCTTATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCG870.18212648370281984No Hit
CTTATACACATCTCCGAGCCCACGAGACAGGTTCCCATCTCGTATGCCGT850.1779396679855136No Hit
CTTTATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCG810.16956603655090122No Hit
CTTATACACATCTCCGAGCCCACGAGACCGGTACCCATCTCGTATGCCGT770.1611924051162888No Hit
CTTATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGAATGCCGT760.1590989972576357No Hit
TCATTATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCC640.13397810295379847No Hit
CTTAATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCG640.13397810295379847No Hit
CTTATACACATCTCCGAGCCCACGAGACCGGTTCCCCTCTCGTATGCCGT640.13397810295379847No Hit
CTAATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTAAGCCGT630.1318846950951454No Hit
CTAATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGACGT610.12769787937783916No Hit
CTTATACACATCTCCGAGCCCACGAGACCGGTTCCCATCACGTATGCCGT610.12769787937783916No Hit
TATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCGTCT590.12351106366053298No Hit
CTTATACACATCTCCGAGCCCACGAGACCGGTTCCCAACTCGTATGCCGT580.1214176558018799No Hit
TCTTATACACATCTCCGAGCCCACGAGCCCGGTTCCCATCTCGTATGCCG580.1214176558018799No Hit
TTATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCGTC550.11513743222592059No Hit
ATTATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCGT550.11513743222592059No Hit
CTAATACACATCTCCGAGCCCACGAGACCGGATCCCATCTCGTATGCCGT540.11304402436726749No Hit
TCTTATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCAG540.11304402436726749No Hit
CTAATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCGA540.11304402436726749No Hit
CTTATACCCATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCGT540.11304402436726749No Hit
CTAATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGAATGCCGT500.10467039293265508No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAATCGT150.002205393370.0513737
CCAAATC150.002205393370.0513735
CAATATC150.002205393370.0513735
ATCACGA150.002205393370.0513737
CGAAAGC309.9829776E-870.0513741
AATATCG150.002205393370.0513736
AATCGTA150.002205393370.0513738
CAAATCG150.002205393370.0513736
CACGAAT207.822622E-570.0513639
AACTCCG353.592504E-970.0513610
TCGAAAG252.787272E-670.0513540
ACACAAC455.456968E-1269.978026
TTAAACA455.456968E-1269.978022
AGGGGCG150.0022145969.9780170
CTTTACA207.8634024E-569.978011
TACAGCA150.0022145969.9780152
TTACACA207.8634024E-569.978013
AAGGGGC150.0022145969.9780169
GAATGCA150.0022145969.9780142
CCGTAAT150.0022145969.9780147
Produced by FastQC (version 0.11.5)