FastQCFastQC Report
Wed 25 May 2016
SRR1780794_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780794_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences397425
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG314957.924765679058941No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGACCGT150.002224543669.9943439
TACCGTG252.3900818E-455.9954727
TCCGTAT503.4770528E-641.99660513
GCCGTAT556.686085E-638.18353754
GAATACG400.002415509735.00597852
GCCACGA1201.8189894E-1235.00597870
TGGTGAC501.7080217E-435.00157541
CCGTGCG501.709286E-434.997179
CCGTATT400.002418494634.9971714
GAACCTA851.06043444E-732.9468041
TCCGAAC552.989167E-431.81961425
GTCCGAA552.989167E-431.81961424
CGTGCGA552.9913752E-431.8156110
TACCACG450.00430415431.11251320
CGGTGGT802.459963E-630.63023246
TCGCCGT703.4810946E-530.00512552
TGGTCGC703.4810946E-530.00512549
CCGGTGG500.00720762627.99773617
ACCGTGC755.5820812E-527.9977368
CCAGTGT500.007212055427.99421531
Produced by FastQC (version 0.11.5)