FastQCFastQC Report
Wed 25 May 2016
SRR1780794_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780794_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences397425
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGTATGCCGT47001.1826130716487389No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTAGTATGCCGT11140.28030445996099895No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGTATGCCG6320.1590237151663836No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGTATTCCGT4930.12404856262187834No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4070.10240925960873119No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGACTT207.919492E-570.0049147
TACGTAA550.063.63281246
TAAGACG650.059.22001643
TCTCGGA601.8189894E-1258.33008238
CGTATGC8400.057.48956341
TCGGATG553.6379788E-1157.26232540
ATCTCGT12150.057.0338637
TCTCGTA11750.056.29473538
CTCGTAT10300.056.0648439
TTACGTA509.804353E-1055.9968845
GGCCCGT252.3912718E-455.989831
CATCTCG13750.055.74234836
TCGTATG9300.055.68881240
GCCGTCA4800.055.41357846
TATGCCG11000.055.35358443
CCGTCTT4500.055.22609347
AGGGGGG11100.054.23121370
CCGTCAT5400.053.80006847
TGCCGTC8600.053.71793745
GCCGTCT4500.053.66367746