FastQCFastQC Report
Wed 25 May 2016
SRR1780793_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780793_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43669
Sequences flagged as poor quality0
Sequence length76
%GC90

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2962167.8307266023953No Hit
CTTATACACATCTGACGCTGCCGACGAGCCACGTCGTGTAGATCTCGGTG1530.35036295770455017No Hit
GGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG770.17632645583823764No Hit
GGGGGGGGGGGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG560.1282374224278092No Hit
GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG530.1213675605120337No Hit
GGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG490.11220774462433304No Hit
GGGGGGGGGGGGGGGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGG470.10762783668048272No Hit
GGGGGGGGGGGGGGGGGGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGG450.1030479287366324No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGGC207.843616E-570.019
CTGCCGG150.002210554270.018
CATTAAA453.7107384E-1062.22221861
TGGTCGC401.0355507E-861.2549
GTATCAT401.0355507E-861.2557
TATCATT401.0355507E-861.2558
ATCATTA401.0355507E-861.2559
TCATTAA401.0355507E-861.2560
GTCGCCG352.9096736E-760.051
CCGTATC352.9096736E-760.055
TCGCCGT352.9096736E-760.052
CGTATCA352.9096736E-760.056
GCCGTAT352.9096736E-760.054
GGTCGCC352.9096736E-760.050
GCCGGCG308.246387E-658.33333220
CGCCGTA308.246387E-658.33333253
CCGGCGG308.246387E-658.33333221
ACGTCGT509.458745E-1055.99999631
GTCGTGT509.458745E-1055.99999633
TCGTGTA509.458745E-1055.99999634