FastQCFastQC Report
Wed 25 May 2016
SRR1780793_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780793_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43669
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCCGT1528935.01110627676384No Hit
TCTTATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCCG28496.5240788660147935No Hit
CTAATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCCGT14493.318143305319563No Hit
CTTATACACATCTCCGAGCCCACGAGACGCCGCTCCATCTCGTATGCCGT12012.7502347202821227No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCC7561.7312052027754241No Hit
CATATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCCGT4961.1358171700748816No Hit
ATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCCGTCTT4320.989260115871671Illumina PCR Primer Index 11 (95% over 22bp)
CTTATACACATCTCCGCGCCCACGAGACGACGCTCCATCTCGTATGCCGT4150.9503308983489432No Hit
TCTTTATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCC3990.9136916347981406No Hit
TCATATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCCG3700.8472829696123109No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3340.7648446266230049No Hit
TCTTATACACATCTCCGAGCCCACGAGACGCCGCTCCATCTCGTATGCCG2220.5083697817673866No Hit
CTAATACACATCTCCGAGCCCACGAGACGCCGCTCCATCTCGTATGCCGT1640.37555245139572696No Hit
CTATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCCGTC1520.34807300373262495No Hit
CAAATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCCGT1430.32746341798529854No Hit
CTTATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGACGT1370.3137236941537475No Hit
CTTATACACATCTCCGAGCCCACGAGACGACGCTCAATCTCGTATGCCGT1140.26105475279946877No Hit
CTTAAACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCCGT1080.24731502896791774No Hit
CTTTACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCCGTC1030.23586525910829192No Hit
TCTTATACACATCTCCGCGCCCACGAGACGACGCTCCATCTCGTATGCCG1020.23357530513636676No Hit
CTTATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCCGA930.2129657193890403No Hit
TCTTAATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCC860.19693604158556416No Hit
CCTTATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCCG840.19235613364171378No Hit
CTTATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCAGT830.19006617966978864No Hit
CTTATACACATCTCCGAGCCCACGAGACGACGCTCCATATCGTATGCCGT790.18090636378208796No Hit
CTTTATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCCG760.17403650186631248No Hit
CTTATACACATCTCCGAGCCCACGAGACGAAGCTCCATCTCGTATGCCGT740.16945659392246215No Hit
CTTAATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCCG710.16258673200668666No Hit
CTTATACACATCTCCGAGCCAACGAGACGACGCTCCATCTCGTATGCCGT710.16258673200668666No Hit
CTTATACACATCTCCGCGCCCACGAGACGCCGCTCCATCTCGTATGCCGT640.14655705420321052No Hit
CTTATACACATCTCCGAGCCCACGAGCCGACGCTCCATCTCGTATGCCGT610.139687192287435No Hit
CTTATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTAAGCCGT610.139687192287435No Hit
TTATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCCGTC590.1351072843435847No Hit
TATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCCGTCT580.13281733037165952Illumina PCR Primer Index 11 (95% over 21bp)
TCATTATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCC570.13052737639973438No Hit
CTAATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCCGA560.1282374224278092No Hit
CTAATACACATCTCCGCGCCCACGAGACGACGCTCCATCTCGTATGCCGT550.12594746845588403No Hit
CTTATACACATCTCCGAGCCCACGAGACGACGCACCATCTCGTATGCCGT540.12365751448395887No Hit
CTTATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGAATGCCGT540.12365751448395887No Hit
CTTATACACATCTCCGAGCCCACGAGACGACGCTCCATCACGTATGCCGT500.11449769859625822No Hit
CTAATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGACGT490.11220774462433304No Hit
CTTATACACATCTCCGAGCCCACGAGACGACGCTCCAACTCGTATGCCGT470.10762783668048272No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGCCGCTCCATCTCGTATGCC470.10762783668048272No Hit
CTTATACACATCTCCGAGCCCACGAGACGACGCTCCCTCTCGTATGCCGT450.1030479287366324No Hit
CTTATACACATCTCCGAGCCCACGAGACGACGCTACATCTCGTATGCCGT440.10075797476470723No Hit
CTTATACACATCACCGAGCCCACGAGACGACGCTCCATCTCGTATGCCGT440.10075797476470723No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTTTAT850.070.000011
TTATGCC150.002210554270.042
CTTTACA252.795443E-670.01
TGCAAGA150.002210554270.055
TTACACA301.00137186E-770.03
AGGCGGG150.002210554270.070
GTCTGCC150.002210554270.042
ACACATA301.00137186E-770.06
TCTGCCG150.002210554270.043
TATCCGA252.795443E-670.011
AAGACGC252.795443E-670.027
ATAAAGG252.795443E-670.067
CGAATGA150.002210554270.041
TCCATAA150.002210554270.034
CTGCCGT150.002210554270.044
TCTTCTA150.002210554270.050
ATCCGAG252.795443E-670.012
TTTACAC301.00137186E-770.02
TTCAGCA150.002210554270.053
TAAAGGG252.795443E-670.068
Produced by FastQC (version 0.11.5)