FastQCFastQC Report
Wed 25 May 2016
SRR1780792_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780792_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences331385
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG298559.009158531617304No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTCGG200.006944969352.50520742
GGACACG200.006944969352.5052071
TGTCGGT200.006944969352.50520743
TCGGTGG757.548806E-1041.99782645
CGAATTC350.001253864340.00396714
TCGAATT400.00241578235.0034713
TCCACCG400.002417571434.99818830
CCGAAAT601.2143701E-534.99818847
AAGGATC400.002417571434.99818827
CGGTGGT652.1002747E-532.3060246
TGCTATT652.1002747E-532.3060223
GTTGGCG552.9871525E-431.82133757
GCGTGTG450.00430516931.10958
GTACAAG703.48107E-530.0029751
CTCGGTG703.48107E-530.00297544
TCGCCGT604.973841E-429.1695652
ATTAGAG604.973841E-429.169563
GCATGTA604.973841E-429.169562
GCGAACT604.978238E-429.16515728
TTGTACG604.978238E-429.1651579