FastQCFastQC Report
Wed 25 May 2016
SRR1780792_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780792_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences331385
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCCGT172225.196976326629148No Hit
TCTTATACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCCG27770.8379980988880005No Hit
CTAATACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCCGT16710.5042473256182386No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCC11370.343105451363218No Hit
CATATACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCCGT5920.17864417520406778No Hit
TCATATACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCCG4350.13126725711785384No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4040.12191257902439762No Hit
ATACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCCGTCTT3980.12010199616759962TruSeq Adapter, Index 9 (95% over 21bp)
CTTATACACATCTCCGCGCCCACGAGACGGAGTAAGATCTCGTATGCCGT3850.11617906664453732No Hit
TCTTTATACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCC3470.10471204188481677No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTCG207.922166E-569.9966825
TCGGAAG150.002223921769.9966744
CTCGGAA150.002223921769.9966743
AGGGGGG25050.060.3746470
ATCGTAA308.369343E-658.33056339
TGCCGTA252.3891054E-455.99734545
TGCCGAC1000.055.99734545
CCGACTT700.054.99739547
CGACTTC653.6379788E-1253.85172748
GATCTCG34000.053.52687536
GCCGACT850.053.5268746
ATCTCGT33300.053.4959637
CGTCTTC32900.053.09060348
TATGCCG34100.053.06199643
TCGTATG34500.052.9540140
CCGTCTT32800.052.9243247
CGTATGC34250.052.8296141
TCTCGTA34200.052.80451238
CTGCTTG32250.052.74964554
CTCGTAT34550.052.6747839