FastQCFastQC Report
Wed 25 May 2016
SRR1780791_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780791_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences268763
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG127324.737259220949312No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTAT501.4551915E-1162.99212654
GTCGCCG452.6959242E-854.43764551
CAGGTAC200.006939482452.5129781
CGCTGTA200.00694970552.4934426
GATTGAC200.00694970552.4934456
CGGTCTA200.00694970552.493447
CGGTGGT703.8198777E-1045.01112746
CGTATCA703.8380676E-1044.99437756
TCGGTGG551.3050521E-744.5564745
TCGCCGT551.308872E-744.5398952
CGCCGTA551.308872E-744.5398953
TCTCGGT659.95351E-943.08757443
AGATCTC659.969881E-943.07955640
CTCGGTG654.8422044E-737.70162644
GATCTCG654.84928E-737.6946141
ATCTCGG654.856356E-737.687642
TGGTCGC708.6502223E-735.00865649
GTACCTA400.002417629134.9956256
ATTTCGG400.002417629134.99562553
GTAGATC957.579729E-933.16609638