FastQCFastQC Report
Wed 25 May 2016
SRR1780791_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780791_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences268763
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCATCCTGTATCTCGTATGCCGT37921.4109084955890507No Hit
TCTTATACACATCTCCGAGCCCACGAGACCATCCTGTATCTCGTATGCCG9720.36165692450225667No Hit
CTAATACACATCTCCGAGCCCACGAGACCATCCTGTATCTCGTATGCCGT5620.20910616416694264No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3620.13469115912532603No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACTCGT150.002222974670.0005537
TAACTCG150.002222974670.0005536
ACGTAAC150.00222461669.987533
GCCGACT308.37154E-658.32294546
TGCCGTA308.37154E-658.32294545
GCCGTAT252.389833E-455.9900346
TTCCGTA200.006951165452.4906545
GCCGTCA1950.050.2474646
TCGTTGT352.085364E-549.99109334
CGTATGA651.8553692E-1048.46192641
CGTCTTA1200.046.65835648
CCGACTT305.876479E-446.65835647
TCGTATG9250.046.1625340
ATGCCGT9100.044.99198544
CGTATGC8900.044.83181841
AGGGGGG4550.044.61573870
TGCCGTC8800.044.5375245
CTCGTAT9600.044.4795239
TATGCCG9250.044.2623943
CATCGTT404.590191E-543.7422132
Produced by FastQC (version 0.11.5)