FastQCFastQC Report
Wed 25 May 2016
SRR1780790_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780790_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences498716
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG207234.155270735248117No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATAT200.006953087652.49360726
GTACGTT200.006955845352.4883463
AACGACG350.001255836939.9951326
TCGGTCA1450.036.20248829
TTCGGTC1500.034.9922328
GTGAACA400.002420759334.992234
AAATGCG957.6433935E-933.150535
GCGAAGT751.4886755E-632.6692432
AATGCGT751.4921789E-632.6594126
ACGTTAG552.9879005E-431.82388170
GTACGCG901.7525781E-731.11668469
CAAGTGT703.4826604E-530.00537364
CCACGTC2200.028.64149370
TGTCGGA755.5788223E-528.00220735
GTCGGAT906.162596E-627.2243736
GCCGAGT906.1674036E-627.22163858
CCGAGTT906.1674036E-627.22163859
TGAAGTT1800.027.2161839
AGACGTT657.9400075E-426.93060168
GGTCACC1900.025.7889231
Produced by FastQC (version 0.11.5)