FastQCFastQC Report
Wed 25 May 2016
SRR1780790_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780790_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences498716
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGCCGT101532.0358280063202305No Hit
TCTTATACACATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGCCG20560.4122586802909872No Hit
CTAATACACATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGCCGT11180.22417568315434033RNA PCR Primer, Index 19 (95% over 21bp)
CTCTTATACACATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGCC5250.10527033421827252No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG16350.055.44930370
TCGTATG24000.050.75029440
GCCGTCT21550.050.66806446
CTCGTAT23800.050.59359739
CCGTCTT21050.050.5415447
CGTATGC23300.050.4723941
TATGCCG22800.050.34107243
TCTCGTA23650.050.02644338
ATCTCGT23400.049.9625637
AATCTCG23450.049.8560336
CGTCTTC21600.049.74066548
GTATGCC23250.049.66774442
AAAACGG850.049.40709367
CTTGAAA22200.049.3471357
TGCCGTC22950.049.10210845
AAGCCGT506.196751E-848.9953744
TCTTCTG21500.048.84238450
ATGCCGT22950.048.79712744
TGCCGAC800.048.1204545
GTCTTCT22150.047.55757549
Produced by FastQC (version 0.11.5)