FastQCFastQC Report
Wed 25 May 2016
SRR1780789_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780789_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences264715
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG243439.19592769582381No Hit
CTTATACACATCTGACGCTGCCGACGAGCCACGTCGTGTAGATCTCGGTG2880.10879625257352248No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGCCG1050.056.66467751
TCGCCGT1000.056.00861452
GCCGTAT1100.054.09922854
TGGTCGC1100.054.0890149
TCTCGGT1100.050.90730343
CGGTGGT1150.048.69394746
GATCTCG950.047.8930641
AGATCTC1100.047.72559740
GTTGGCG305.866707E-446.6738557
GTCTAAG305.872174E-446.665031
TCGGTGG1200.046.6650345
CTCGGTG1150.045.65057444
ATCTCGG1000.045.498442
GCCACGA551.305907E-744.55230770
CGTATCA1200.043.75673356
CGCCGTA1300.043.0835553
GTAGATC1100.041.36218338
TAGATCT1300.040.38319839
CTATCGG350.001254235439.99859638
GTATCAT1450.038.6266357
Produced by FastQC (version 0.11.5)