##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780789_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 264715 Sequences flagged as poor quality 0 Sequence length 76 %GC 51 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 27.39170428574127 32.0 27.0 32.0 13.0 32.0 2 28.58775664393782 32.0 27.0 32.0 13.0 32.0 3 28.31038286459022 32.0 27.0 32.0 13.0 32.0 4 27.31675575619062 32.0 27.0 32.0 13.0 32.0 5 27.891245301550725 32.0 27.0 32.0 13.0 32.0 6 31.633802391250967 37.0 32.0 37.0 13.0 37.0 7 31.49662467181686 37.0 32.0 37.0 13.0 37.0 8 31.437194718848573 37.0 32.0 37.0 13.0 37.0 9 31.44982717261961 37.0 32.0 37.0 13.0 37.0 10-11 31.161460438584893 37.0 29.5 37.0 13.0 37.0 12-13 31.188668945847418 37.0 27.0 37.0 13.0 37.0 14-15 31.795362937498822 37.0 32.0 37.0 13.0 37.0 16-17 31.71814593052906 37.0 29.5 37.0 13.0 37.0 18-19 31.168428309691556 37.0 27.0 37.0 13.0 37.0 20-21 31.561766428045257 37.0 29.5 37.0 13.0 37.0 22-23 31.3729539315868 37.0 29.5 37.0 13.0 37.0 24-25 30.904712615454358 37.0 27.0 37.0 13.0 37.0 26-27 31.34246264850877 37.0 29.5 37.0 13.0 37.0 28-29 31.22927488053189 37.0 29.5 37.0 13.0 37.0 30-31 31.142915966227832 37.0 29.5 37.0 13.0 37.0 32-33 31.293704550176606 37.0 29.5 37.0 13.0 37.0 34-35 31.121373930453508 37.0 29.5 37.0 13.0 37.0 36-37 31.66966737812364 37.0 32.0 37.0 13.0 37.0 38-39 31.718416032336663 37.0 32.0 37.0 13.0 37.0 40-41 31.21214135957539 37.0 29.5 37.0 13.0 37.0 42-43 31.021672364618553 37.0 27.0 37.0 13.0 37.0 44-45 30.621156715713127 37.0 27.0 37.0 13.0 37.0 46-47 30.821406040458605 37.0 29.5 37.0 13.0 37.0 48-49 31.36509831328032 37.0 29.5 37.0 13.0 37.0 50-51 31.583504901497836 37.0 32.0 37.0 13.0 37.0 52-53 30.553510379086944 37.0 27.0 37.0 13.0 37.0 54-55 30.554035472111515 37.0 27.0 37.0 13.0 37.0 56-57 30.24537899250137 37.0 27.0 37.0 13.0 37.0 58-59 29.970948000679975 37.0 27.0 37.0 13.0 37.0 60-61 29.762302854012805 37.0 24.5 37.0 13.0 37.0 62-63 29.579483595565044 37.0 24.5 37.0 10.5 37.0 64-65 29.689152484747748 37.0 24.5 37.0 13.0 37.0 66-67 29.828870672232398 37.0 27.0 37.0 13.0 37.0 68-69 29.99838505562586 37.0 27.0 37.0 13.0 37.0 70-71 30.00985021627033 37.0 27.0 37.0 13.0 37.0 72-73 30.157667680335457 37.0 27.0 37.0 13.0 37.0 74-75 30.309070132028786 37.0 27.0 37.0 13.0 37.0 76 29.42345541431351 37.0 22.0 37.0 13.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 11101 1 -0.7590500484351459 11101 2 -1.0463349736515468 11101 3 0.04134667851128171 11101 4 0.04077457553588104 11101 5 -0.6721950976768021 11101 6 -1.4645669868697482 11101 7 -0.32064849297495357 11101 8 -1.2884844505549502 11101 9 -0.5570482013720088 11101 10-11 -1.67617701697392 11101 12-13 -0.44042726410124544 11101 14-15 -0.8895263136291192 11101 16-17 -1.8270389855704359 11101 18-19 -0.6405232334265989 11101 20-21 -0.7830046045690082 11101 22-23 0.17120045168497455 11101 24-25 -0.43059265283216774 11101 26-27 -0.5192848819505151 11101 28-29 -0.9472209503027145 11101 30-31 -0.8444965320115436 11101 32-33 -0.3709632952321904 11101 34-35 -1.347948256971975 11101 36-37 -0.5571095133339803 11101 38-39 -0.47947647938299554 11101 40-41 -0.626757978070863 11101 42-43 -1.2291767390314376 11101 44-45 -0.48763637636740853 11101 46-47 -2.1656561434018577 11101 48-49 -0.46326869114218283 11101 50-51 0.014297319591236146 11101 52-53 -0.7035940690799904 11101 54-55 0.00347183376443283 11101 56-57 -0.8809086011751113 11101 58-59 -1.3507993496420276 11101 60-61 -1.6404615588030431 11101 62-63 -1.3528440874631116 11101 64-65 -1.152093954133317 11101 66-67 -1.8895253165270312 11101 68-69 -1.803813485150819 11101 70-71 -2.0690384706160074 11101 72-73 -0.5307913299619429 11101 74-75 -1.3223860739758635 11101 76 -1.271373536706097 11102 1 -0.10722482719620885 11102 2 -0.10129902232411325 11102 3 -1.3752047595418162 11102 4 -1.1080777474729686 11102 5 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12-13 1.8426460822314397 21610 14-15 0.9101774511826406 21610 16-17 1.2945674341085827 21610 18-19 1.1208386984768062 21610 20-21 1.1817846559679595 21610 22-23 1.0621746529749139 21610 24-25 0.815451294970444 21610 26-27 1.8793201877960009 21610 28-29 0.9858448964049522 21610 30-31 1.0557209571140014 21610 32-33 1.0498781626949132 21610 34-35 0.7288104050949258 21610 36-37 1.3591009261440448 21610 38-39 1.77762941532227 21610 40-41 1.920114678296322 21610 42-43 2.116405981832518 21610 44-45 1.5873973719451762 21610 46-47 1.516059770802432 21610 48-49 1.076291830831714 21610 50-51 1.3843288180163142 21610 52-53 1.3580153504490369 21610 54-55 1.44030624204402 21610 56-57 2.225942306279517 21610 58-59 1.4708783164746677 21610 60-61 1.3201091626608807 21610 62-63 1.0710159695340415 21610 64-65 1.3581430340172744 21610 66-67 1.7245439800081463 21610 68-69 1.5259450269235764 21610 70-71 1.4150435752816968 21610 72-73 1.1182074700980564 21610 74-75 1.28101500597014 21610 76 1.2788739509195217 21611 1 0.44902517280378973 21611 2 0.3846384776758853 21611 3 0.3076077404581845 21611 4 -0.4252652474729679 21611 5 -0.031560879387715346 21611 6 0.8901159039857056 21611 7 1.4085181736917107 21611 8 -0.3150331231213208 21611 9 0.40534117915896317 21611 10-11 0.2593871423181149 21611 12-13 -0.24739629064991675 21611 14-15 0.09806586926173821 21611 16-17 0.20120585218767317 21611 18-19 1.3026183594937528 21611 20-21 1.1568552774368897 21611 22-23 0.9356916021274522 21611 24-25 0.9802111819760952 21611 26-27 0.9836280974005156 21611 28-29 0.6397996986648344 21611 30-31 0.4610528780179557 21611 32-33 0.8108951118474543 21611 34-35 1.3323061678067916 21611 36-37 0.6217068583474372 21611 38-39 0.9361393023279199 21611 40-41 0.7981867121946244 21611 42-43 1.447408806691275 21611 44-45 0.4519100838095831 21611 46-47 1.1061727651527136 21611 48-49 0.8016096274418878 21611 50-51 0.8053740157564278 21611 52-53 0.6316382318049705 21611 54-55 1.798180535829335 21611 56-57 1.1157728147540915 21611 58-59 2.005376904045285 21611 60-61 1.779007467745629 21611 62-63 0.9106699243363003 21611 64-65 0.9624156328873319 21611 66-67 1.265010081703057 21611 68-69 1.5701540664715985 21611 70-71 0.5844644792364981 21611 72-73 0.7751636847873229 21611 74-75 0.4657960794164673 21611 76 0.6644671712585044 21612 1 0.12402517280379044 21612 2 0.04297181100922032 21612 3 0.6117744071248516 21612 4 -0.03776524747296861 21612 5 0.7434391206122832 21612 6 -0.2015507626809594 21612 7 0.3251848403583786 21612 8 -0.5733664564546537 21612 9 0.4261745124922953 21612 10-11 0.532303808984782 21612 12-13 0.32968704268341753 21612 14-15 1.1126492025950725 21612 16-17 -0.03004414781232967 21612 18-19 0.29845169282708994 21612 20-21 0.9089386107702211 21612 22-23 0.14402493546078787 21612 24-25 -0.1552054846905726 21612 26-27 0.6982114307338527 21612 28-29 0.30646636533150584 21612 30-31 0.7860528780179585 21612 32-33 0.33797844518078435 21612 34-35 0.2198061678067873 21612 36-37 0.8279568583474344 21612 38-39 0.4944726356612499 21612 40-41 0.454436712194628 21612 42-43 0.534908806691277 21612 44-45 0.5164934171429181 21612 46-47 0.47492276515271215 21612 48-49 1.5661929607752185 21612 50-51 1.2116240157564278 21612 52-53 0.7878882318049705 21612 54-55 1.9023472024959922 21612 56-57 0.9866061480874251 21612 58-59 0.8637102373786227 21612 60-61 0.5040074677456268 21612 62-63 0.9960865910029675 21612 64-65 0.5249156328873319 21612 66-67 1.3087600817030598 21612 68-69 0.6659873998049335 21612 70-71 0.6094644792365003 21612 72-73 0.21474701812065433 21612 74-75 0.8116294127498023 21612 76 1.689467171258503 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 1.0 9 1.0 10 0.0 11 20.0 12 75.0 13 172.0 14 395.0 15 674.0 16 1138.0 17 1713.0 18 2419.0 19 3264.0 20 4370.0 21 5396.0 22 6184.0 23 7051.0 24 7614.0 25 8372.0 26 9224.0 27 10193.0 28 11396.0 29 13135.0 30 15525.0 31 18834.0 32 23610.0 33 30465.0 34 37362.0 35 32469.0 36 13643.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 48.23877416868781 19.770534412282494 10.05319189125885 21.93749952777085 2 29.776431210475018 22.253367887392052 28.38469849568956 19.585502406443375 3 27.888702091085964 22.99854549575927 26.891327754585465 22.221424658569298 4 23.821270583613195 19.064798479863097 31.5893061542123 25.524624782311413 5 23.47523544366918 30.550822966760226 31.790278528066246 14.183663061504346 6 35.17989633860707 33.77910754491742 17.784502168427096 13.256493948048414 7 34.7439602576453 28.741429138097125 19.812621597627547 16.701989006630022 8 32.51949288283115 32.0877028619783 18.785321405905286 16.60748284928526 9 31.612808172085256 19.43665802815199 19.50994688446805 29.440586915294702 10-11 33.34038510213102 24.595499208564878 23.574566788435604 18.489548900868503 12-13 32.575574410855815 23.706300574977522 22.97586756628108 20.742257447885578 14-15 31.514138380461272 23.77590147522714 21.023592300863225 23.686367843448366 16-17 31.157360271853392 26.40294822658731 22.14286658745795 20.296824914101354 18-19 30.030562670474726 26.78068167222008 23.502655816729757 19.686099840575437 20-21 30.757723147247436 26.326983557322702 23.163869554130503 19.75142374129936 22-23 31.515858984239237 26.057580428245465 22.80361757105943 19.622943016455867 24-25 30.6089219906651 26.497371607314545 22.885999724221822 20.007706677798534 26-27 30.61729467741296 26.025503236542423 23.685597338989805 19.671604747054815 28-29 30.984356524662743 25.29381098632093 24.479526729149903 19.242305759866422 30-31 30.697847488232583 25.634269437959457 23.654585703816196 20.013297369991765 32-33 30.346359513506265 26.151966015703948 23.578777570213497 19.92289690057629 34-35 30.837841513539654 25.685293762088975 23.91463672630561 19.562227998065765 36-37 31.472691039941065 25.01336405965187 24.0165846563595 19.497360244047563 38-39 30.74883077829743 25.286166539481535 24.68927791587648 19.27572476634455 40-41 30.837666765518662 24.53896877851046 24.76506910509982 19.858295350871064 42-43 30.28038124596258 24.55980295492065 24.896774217920676 20.2630415811961 44-45 29.740409625036122 24.675535642440373 25.091658465728894 20.492396266794607 46-47 29.930337019225323 24.29742012746361 25.06582849457694 20.70641435873413 48-49 29.513352398293975 23.503356516023256 26.73785175419045 20.245439331492317 50-51 29.60214275865065 23.967012396795685 25.936937464465228 20.493907380088437 52-53 28.452698465467346 24.78334454073576 25.80202186576806 20.961935128028834 54-55 28.263596113453314 25.11314353929613 25.248198014415884 21.37506233283467 56-57 28.17632492080792 25.19262045035124 25.147664660026557 21.483389968814283 58-59 28.09894406770056 24.86219989043994 25.610230642815317 21.428625399044183 60-61 28.07487439084281 25.308261871482003 25.005666578519893 21.611197159155303 62-63 28.062808830862686 24.580481133928707 25.093688139383772 22.263021895824835 64-65 28.61039436949615 24.287592415593444 24.59076467410399 22.51124854080642 66-67 28.747473602689787 24.069246897489656 23.91076859145086 23.272510908369696 68-69 29.018584874832126 23.65789021342667 23.633712309386503 23.689812602354703 70-71 29.909518502427325 23.52065584918491 22.98135590018701 23.58846974820076 72-73 30.285314923075468 23.266435594004705 22.260986182862215 24.18726330005761 74-75 30.942801772499063 22.832636346808403 21.87026606374499 24.354295816947538 76 31.513871679410077 22.168620991870412 21.436126809513738 24.881380519205777 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.5 2 2.5 3 2.5 4 1.0 5 2.5 6 4.5 7 7.0 8 9.0 9 13.5 10 16.5 11 20.5 12 26.0 13 43.5 14 53.0 15 72.5 16 135.0 17 170.0 18 218.0 19 288.0 20 366.5 21 423.0 22 550.5 23 742.5 24 938.5 25 1070.0 26 1295.5 27 1778.5 28 2305.5 29 2575.0 30 2998.5 31 3747.5 32 4660.0 33 5247.0 34 5773.5 35 7004.5 36 8335.5 37 8962.0 38 9790.5 39 11338.5 40 12940.5 41 14064.0 42 14305.0 43 14490.0 44 14933.5 45 14899.5 46 14607.0 47 14580.0 48 13713.5 49 12109.0 50 11344.0 51 10500.5 52 8885.5 53 7433.5 54 6753.0 55 6280.5 56 5097.5 57 3887.0 58 3387.0 59 2925.0 60 2163.5 61 1691.5 62 1519.0 63 1414.5 64 1207.5 65 1071.5 66 961.5 67 885.0 68 799.5 69 691.5 70 648.0 71 627.0 72 582.5 73 512.5 74 471.5 75 456.0 76 448.5 77 400.0 78 358.0 79 357.0 80 319.5 81 277.0 82 241.5 83 211.0 84 208.0 85 235.0 86 241.5 87 218.0 88 238.0 89 257.5 90 284.5 91 326.0 92 340.0 93 363.5 94 450.5 95 593.0 96 672.0 97 844.5 98 1451.5 99 13329.5 100 24773.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.004910941956443722 2 0.0033998828929225774 3 0.007555295317605727 4 7.555295317605727E-4 5 7.555295317605727E-4 6 0.0026443533611620043 7 0.0037776476588028637 8 0.001133294297640859 9 0.0033998828929225774 10-11 0.002266588595281718 12-13 0.0033998828929225774 14-15 0.0037776476588028637 16-17 0.005099824339383866 18-19 0.004910941956443722 20-21 0.0028332357441021474 22-23 0.0026443533611620043 24-25 0.0035887652758627205 26-27 0.0013221766805810021 28-29 0.0015110590635211453 30-31 3.777647658802863E-4 32-33 0.0016999414464612887 34-35 0.0041554124246831504 36-37 0.004722059573503579 38-39 0.0033998828929225774 40-41 0.0035887652758627205 42-43 0.0015110590635211453 44-45 0.0035887652758627205 46-47 0.004533177190563436 48-49 0.002266588595281718 50-51 0.0035887652758627205 52-53 0.0033998828929225774 54-55 0.0026443533611620043 56-57 0.003966530041743007 58-59 0.007555295317605727 60-61 0.0018888238294014318 62-63 0.0026443533611620043 64-65 0.0052887067223240086 66-67 0.0037776476588028637 68-69 0.003966530041743007 70-71 0.007555295317605727 72-73 0.0028332357441021474 74-75 0.0015110590635211453 76 0.001133294297640859 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 264715.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.662297050513295 #Duplication Level Percentage of deduplicated Percentage of total 1 78.41371772949049 41.29446495905529 2 9.15433945100816 9.64177086940449 3 4.684708332219902 7.401225053591376 4 2.440938185419945 5.141816472101041 5 1.367199535972405 3.5999934045351365 6 0.8136803972517521 2.5710167270550857 7 0.5647432880087132 2.0818475153278904 8 0.4520716921062941 1.9045706990263878 9 0.33522928349507053 1.5888549696703325 >10 1.7546292289706666 14.845546954873726 >50 0.017301116359953825 0.578179364061793 >100 7.208798483314095E-4 0.10933398348640602 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 7.208798483314095E-4 9.24137902781105 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 24343 9.19592769582381 No Hit CTTATACACATCTGACGCTGCCGACGAGCCACGTCGTGTAGATCTCGGTG 288 0.10879625257352248 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.010199648678767732 0.0 2 0.0 0.0 0.0 0.010955178210528304 0.0 3 0.0 0.0 0.0 0.010955178210528304 0.0 4 0.0 0.0 0.0 0.018510473528134033 0.0 5 0.0 0.0 0.0 0.020021532591655178 0.0 6 0.0 0.0 0.0 0.02077706212341575 0.0 7 0.0 0.0 0.0 0.021154826889296034 0.0 8 0.0 0.0 0.0 0.021154826889296034 0.0 9 0.0 0.0 0.0 0.023421415484577754 0.0 10 0.0 0.0 0.0 0.024176945016338325 0.0 11 0.0 0.0 0.0 0.0249324745480989 0.0 12 0.0 0.0 0.0 0.02568800407985947 0.0 13 0.0 0.0 0.0 0.026443533611620045 0.0 14 0.0 0.0 0.0 0.027199063143380615 0.0 15 0.0 0.0 0.0 0.027199063143380615 0.0 16 0.0 0.0 0.0 0.027576827909260904 0.0 17 0.0 0.0 0.0 0.02795459267514119 0.0 18 0.0 0.0 0.0 0.02984341650454262 0.0 19 0.0 0.0 0.0 0.030221181270422906 0.0 20 0.0 0.0 0.0 0.03286553463158491 0.0 21 0.0 0.0 0.0 0.03286553463158491 0.0 22 0.0 0.0 0.0 0.03928753565154978 0.0 23 0.0 0.0 0.0 0.040043065183310356 0.0 24 0.0 0.0 0.0 0.0441984776079935 0.0 25 0.0 0.0 0.0 0.04835389003267665 0.0 26 0.0 0.0 0.0 0.05137600815971894 0.0 27 0.0 0.0 0.0 0.06195342160436696 0.0 28 0.0 0.0 0.0 0.07895283606897985 0.0 29 0.0 0.0 0.0 0.10010766295827588 0.0 30 0.0 0.0 0.0 0.1333509623557411 0.0 31 0.0 0.0 0.0 0.18170485238841774 0.0 32 0.0 0.0 0.0 0.22061462327408723 0.0 33 0.0 0.0 0.0 0.27614604385848934 0.0 34 0.0 0.0 0.0 0.34187711312165914 0.0 35 0.0 0.0 0.0 0.45936195531042817 0.0 36 0.0 0.0 0.0 0.6297338647224373 0.0 37 0.0 0.0 0.0 0.9576336815065258 0.0 38 0.0 0.0 0.0 1.3338873883232911 0.0 39 0.0 0.0 0.0 1.8144041705230154 0.0 40 0.0 0.0 0.0 2.4482934476701357 0.0 41 0.0 0.0 0.0 3.253310163761026 0.0 42 0.0 0.0 0.0 4.121035830988044 0.0 43 0.0 0.0 0.0 5.004627618382034 0.0 44 0.0 0.0 0.0 5.840621045275107 0.0 45 0.0 0.0 0.0 6.723835067903217 0.0 46 0.0 0.0 0.0 7.6300927412500235 0.0 47 0.0 0.0 0.0 8.501218291369964 0.0 48 0.0 0.0 0.0 9.322478892393706 0.0 49 0.0 0.0 0.0 10.147517141076252 0.0 50 0.0 0.0 0.0 10.93742326653193 0.0 51 0.0 0.0 0.0 11.82063728916004 0.0 52 0.0 0.0 0.0 12.698184840299945 0.0 53 0.0 0.0 0.0 13.5364448557883 0.0 54 0.0 0.0 0.0 14.346372513835634 0.0 55 0.0 0.0 0.0 15.122679107719623 0.0 56 0.0 0.0 0.0 15.848365222975653 0.0 57 0.0 0.0 0.0 16.600117107077423 0.0 58 0.0 0.0 0.0 17.31900345654761 0.0 59 0.0 0.0 0.0 17.986513797858073 0.0 60 0.0 0.0 0.0 18.642313431426253 0.0 61 0.0 0.0 0.0 19.33060083486013 0.0 62 0.0 0.0 0.0 20.02417694501634 0.0 63 0.0 0.0 0.0 20.666377047012826 0.0 64 0.0 0.0 0.0 21.450616700980298 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTCGCCG 105 0.0 56.664677 51 TCGCCGT 100 0.0 56.008614 52 GCCGTAT 110 0.0 54.099228 54 TGGTCGC 110 0.0 54.08901 49 TCTCGGT 110 0.0 50.907303 43 CGGTGGT 115 0.0 48.693947 46 GATCTCG 95 0.0 47.89306 41 AGATCTC 110 0.0 47.725597 40 GTTGGCG 30 5.866707E-4 46.67385 57 GTCTAAG 30 5.872174E-4 46.66503 1 TCGGTGG 120 0.0 46.66503 45 CTCGGTG 115 0.0 45.650574 44 ATCTCGG 100 0.0 45.4984 42 GCCACGA 55 1.305907E-7 44.552307 70 CGTATCA 120 0.0 43.756733 56 CGCCGTA 130 0.0 43.08355 53 GTAGATC 110 0.0 41.362183 38 TAGATCT 130 0.0 40.383198 39 CTATCGG 35 0.0012542354 39.998596 38 GTATCAT 145 0.0 38.62663 57 >>END_MODULE