FastQCFastQC Report
Wed 25 May 2016
SRR1780789_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780789_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences264715
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCCGT87863.3190412330241963No Hit
TCTTATACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCCG20380.7698845928640236No Hit
CTAATACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCCGT15790.5964905653249721No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6770.25574674650095386No Hit
ATACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCCGTCTT6700.2531023931397919No Hit
TCATATACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCCG6060.22892544812345353No Hit
TCTTTATACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCC4530.17112743894376972No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCC3770.14241731673686797No Hit
CATATACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCCGT3690.13939519860982566No Hit
CTTATACACATCTCCGAGCCCACGAGACACAAATAAATCTCGTATGCCGT2820.10652966397824075No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTCTA150.002221437470.0124741
AGGGGGG13200.058.6089270
TGACGAC308.362893E-658.33270645
CTCCGGT252.3877918E-455.9993977
CGTTGAA252.3877918E-455.99939742
GACGACT252.3877918E-455.99939746
TCCGGTC252.3877918E-455.9993978
GTTTGAC200.006946452452.49943528
CGTCTTA608.367351E-1152.49943548
ATACCGT200.006946452452.49943544
TACCGTC200.006946452452.49943545
AACTCGT750.051.3327837
CGACTTC900.050.55501648
CGTCTAC1050.049.99946248
ATGACGA352.083215E-549.99946244
CATCTCG850.049.41123236
CCGACTT800.048.1244847
CTCGTAT25200.047.7772639
TATGCCG24900.047.50952543
CTTTATA1400.047.499492