FastQCFastQC Report
Wed 25 May 2016
SRR1780788_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780788_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences68739
Sequences flagged as poor quality0
Sequence length76
%GC82

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3810855.43868837195769No Hit
CTTATACACATCTGACGCTGCCGACGAGCCACGTCGTGTAGATCTCGGTG2500.3636945547651261No Hit
GGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG910.13238481793450588No Hit
GGGGGGGGGGGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG710.1032892535532958No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTTGAA150.00221598770.026
TCGATGT150.00221598770.022
TTCGATG150.00221598770.021
TCGAAGG150.00221598770.067
TCGGACT150.00221598770.046
TAGTTCG150.00221598770.042
ATTCGAT150.00221598770.020
AGGTCGA150.00221598770.064
AGACTAG150.00221598770.038
AGTTCGG150.00221598770.043
TTCGGAC150.00221598770.045
CATAATC150.00221598770.051
GACTAGT150.00221598770.039
AAGCACC150.00221598770.018
GTTCGGA150.00221598770.044
GGTCGAA150.00221598770.065
GTTGAAG200.006924384752.50000427
CTTTAAC200.006924384752.50000435
CCTGCGG200.006924384752.50000410
TCGGTGG407.380895E-752.50000445
Produced by FastQC (version 0.11.5)