FastQCFastQC Report
Wed 25 May 2016
SRR1780788_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780788_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences68739
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCGT1943628.275069465659957No Hit
TCTTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCG31214.540362821687833No Hit
CTAATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCGT15672.27963746926781No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCC10921.5886178152140706No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5570.810311468016701No Hit
CTTATACACATCTCCGCGCCCACGAGACCTTGGGTCATCTCGTATGCCGT5340.7768515689783093No Hit
CATATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCGT5300.7710324561020673No Hit
TCTTTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCC5270.7666681214448857No Hit
TCATATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCG4710.6852005411774975No Hit
ATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCGTCTT4180.6080972955672908TruSeq Adapter, Index 7 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGACGT3220.4684385865374824No Hit
CTTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCACGTATGCCGT3080.4480716914706353No Hit
CTTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTAGTATGCCGT2300.334598990383916No Hit
CTTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTAAGCCGT2110.3069582042217664No Hit
CTTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCGA1960.28513653093585883No Hit
CTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCGTC1900.27640786162149583No Hit
TCTTATACACATCTCCGCGCCCACGAGACCTTGGGTCATCTCGTATGCCG1510.21967151107813612No Hit
CTTATACACATCTCCGAGCCCACGAGACCTAGGGTCATCTCGTATGCCGT1380.20075939423034958No Hit
CTTATACACATCTCCGAGCCCACGAGACCTTGGGTCATATCGTATGCCGT1380.20075939423034958No Hit
CTTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCAGT1340.19494028135410757No Hit
CTTATACACATCTCCGAGCCCACGAGACCATGGGTCATCTCGTATGCCGT1280.18621161203974454No Hit
CTTTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCG1180.1716638298491395No Hit
CAAATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCGT1160.1687542734110185No Hit
TCTTAATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCC1160.1687542734110185No Hit
CCTTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCG1140.1658447169728975No Hit
CTTATACACATCTCCGAGCCCACGAGACCTTGGGACATCTCGTATGCCGT1130.16438993875383698No Hit
CTTTACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCGTC1100.16002560409665545No Hit
CTTATACACATCTCCGAGCCCACGAGACATTGGGTCATCTCGTATGCCGT1050.15275171300135293No Hit
CTTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGAATGCCGT1010.14693260012511092No Hit
CTTAATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCG960.1396587090298084No Hit
CTAATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTAAGCCGT920.1338395961535664No Hit
CTTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATTCCGT850.12365614862014288No Hit
CTAATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGACGT850.12365614862014288No Hit
CTTAAACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCGT840.12220137040108235No Hit
CTAATACACATCTCCGAGCCCACGAGACCTTGGGTCATCACGTATGCCGT830.12074659218202184No Hit
CTAATACACATCTCCGCGCCCACGAGACCTTGGGTCATCTCGTATGCCGT820.11929181396296135No Hit
TTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCGTC740.10765358821047731No Hit
TATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCGTCT730.10619880999141681No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACATAT252.8126433E-670.000017
CTTTAAA207.875757E-570.0000157
ACATATC207.875757E-570.000018
ATGCCAT207.875757E-570.0000144
ATATGCA207.875757E-570.0000152
CTTGAGT207.875757E-570.0000129
ATCCGAG207.875757E-570.0000112
GTAATCC207.875757E-570.0000142
ATATCCG207.875757E-570.0000110
CATATGC252.8126433E-670.0000151
CATATCC207.875757E-570.000019
ATTCAGC207.875757E-570.0000151
TGAGTCA207.875757E-570.0000131
GTCATGA150.00221598770.034
TCGACCT150.00221598770.024
CAACCGA150.00221598770.010
GTCCGAA150.00221598770.043
TTCGACC150.00221598770.023
CGAAGGG150.00221598770.034
ATGTGTT150.00221598770.07
Produced by FastQC (version 0.11.5)