##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780788_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 68739 Sequences flagged as poor quality 0 Sequence length 76 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.078936266166224 32.0 32.0 32.0 27.0 32.0 2 30.175388062089933 32.0 32.0 32.0 27.0 32.0 3 28.339341567378053 32.0 27.0 32.0 13.0 32.0 4 30.097819287449628 32.0 32.0 32.0 27.0 32.0 5 30.267751931218086 32.0 32.0 32.0 27.0 32.0 6 33.502407657952546 37.0 32.0 37.0 22.0 37.0 7 33.87251778466373 37.0 32.0 37.0 27.0 37.0 8 31.9499847248287 37.0 32.0 37.0 13.0 37.0 9 34.16876882119321 37.0 32.0 37.0 27.0 37.0 10-11 33.37526731549775 37.0 34.5 37.0 24.5 37.0 12-13 34.10037969711517 37.0 34.5 37.0 27.0 37.0 14-15 33.86152693521873 37.0 32.0 37.0 27.0 37.0 16-17 30.847917484979416 34.5 27.0 37.0 17.5 37.0 18-19 32.648554677839364 37.0 32.0 37.0 17.5 37.0 20-21 33.91089483408254 37.0 34.5 37.0 27.0 37.0 22-23 32.63066817963601 37.0 29.5 37.0 20.0 37.0 24-25 31.815992376962136 37.0 29.5 37.0 17.5 37.0 26-27 32.752578594393285 37.0 32.0 37.0 17.5 37.0 28-29 33.46141928163051 37.0 37.0 37.0 22.0 37.0 30-31 33.09097455592895 37.0 34.5 37.0 22.0 37.0 32-33 32.47446864225549 37.0 32.0 37.0 13.0 37.0 34-35 33.128020483277325 37.0 34.5 37.0 17.5 37.0 36-37 32.84043265104235 37.0 34.5 37.0 20.0 37.0 38-39 29.653675497170454 37.0 24.5 37.0 13.0 37.0 40-41 29.257423005862755 37.0 24.5 37.0 10.5 37.0 42-43 30.54425435342382 37.0 27.0 37.0 10.5 37.0 44-45 31.736765155152096 37.0 29.5 37.0 13.0 37.0 46-47 29.735113981873464 37.0 24.5 37.0 13.0 37.0 48-49 29.642808303874073 37.0 24.5 37.0 10.5 37.0 50-51 29.6402915375551 37.0 24.5 37.0 13.0 37.0 52-53 28.993700810311466 34.5 20.0 37.0 10.5 37.0 54-55 28.38338497796011 34.5 22.0 37.0 13.0 37.0 56-57 28.543505142641003 34.5 17.5 37.0 10.5 37.0 58-59 28.734510248912557 34.5 20.0 37.0 10.5 37.0 60-61 29.675380788198837 37.0 24.5 37.0 10.5 37.0 62-63 32.28626398405563 37.0 32.0 37.0 13.0 37.0 64-65 32.856849823244445 37.0 32.0 37.0 22.0 37.0 66-67 32.787093207640496 37.0 32.0 37.0 13.0 37.0 68-69 32.182400093105805 37.0 32.0 37.0 13.0 37.0 70-71 27.936506204629104 32.0 20.0 37.0 13.0 37.0 72-73 28.769330365585766 34.5 20.0 37.0 10.5 37.0 74-75 31.5198213532347 37.0 32.0 37.0 13.0 37.0 76 32.48062962801321 37.0 32.0 37.0 13.0 37.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 11101 1 0.21549546191505442 11101 2 -0.03809134613872445 11101 3 -0.6993530479343413 11101 4 -0.17399245796607588 11101 5 -1.2659549390493794 11101 6 -1.3504125323969944 11101 7 -0.7045670074877819 11101 8 -2.1450031617133334 11101 9 0.5203081724332392 11101 10-11 -2.157045644926754 11101 12-13 -1.2900909851992992 11101 14-15 -1.8950317167138877 11101 16-17 -2.3367898049242655 11101 18-19 -0.33533985506532815 11101 20-21 -2.68230836935259 11101 22-23 0.9522966802137205 11101 24-25 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48-49 -0.38936213045825596 21612 50-51 1.6965963397039303 21612 52-53 0.2803742206309856 21612 54-55 1.7631128330708137 21612 56-57 1.7915834023187536 21612 58-59 1.9062336920158671 21612 60-61 1.7515253524790246 21612 62-63 0.6192607585277727 21612 64-65 -0.5905359613115664 21612 66-67 0.7855102122794975 21612 68-69 -0.6501907780831715 21612 70-71 -1.6780184866386527 21612 72-73 0.07338715420572939 21612 74-75 0.6960824982439568 21612 76 -0.009467373657557232 >>END_MODULE >>Per sequence quality scores pass #Quality Count 17 8.0 18 13.0 19 35.0 20 109.0 21 307.0 22 582.0 23 1032.0 24 1449.0 25 2100.0 26 2588.0 27 3247.0 28 4069.0 29 4804.0 30 5836.0 31 6995.0 32 8651.0 33 10700.0 34 10365.0 35 5417.0 36 432.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 14.534926015917126 9.676856149335816 14.825915525745298 60.96230230900176 2 7.892516294227188 13.12703677839851 59.399732309124765 19.580714618249534 3 7.268071982426279 21.890047862203406 58.35988303583118 12.481997119539127 4 5.570345800782671 57.34008350426978 25.775760485314013 11.313810209633541 5 5.833660658432622 25.01927581140255 62.85223817629002 6.294825353874802 6 10.222726545338164 66.49354805859846 16.194591134581533 7.089134261481838 7 9.80126862197393 22.133961824953445 11.961708566108008 56.103060986964614 8 8.763583991620477 65.39373572498872 7.96491074935626 17.877769534034538 9 8.97161727694613 16.840512663844397 7.128413273396471 67.059456785813 10-11 9.221111741515006 40.807983822866206 34.04326510423486 15.927639331383931 12-13 9.738497908710675 11.058010547372248 40.12074922713221 39.082742316784866 14-15 9.875034550982702 9.210200904872053 14.379027917194023 66.53573662695122 16-17 38.924895435533735 33.24749954537188 7.792689579923623 20.03491543917076 18-19 40.75197486143238 16.577197806194448 8.071109559347677 34.5997177730255 20-21 11.625386433897072 10.141480269139844 7.855246408437898 70.37788688852518 22-23 9.891764500501898 39.50304776036893 7.863076274022025 42.74211146510714 24-25 38.83849662125301 36.91526582628367 8.124995453784997 16.121242098678323 26-27 40.74806872572268 41.20706460858053 8.640906644165442 9.403960021531343 28-29 11.329977596089499 16.69382292181908 9.790072449009282 62.18612703308214 30-31 10.4847391544706 10.430184177601909 61.98536471820536 17.09971194972213 32-33 63.586833969812695 9.194398981633025 18.725586470267324 8.493180578286962 34-35 48.09787747857839 9.875034550982702 33.9843465863629 8.042741384075997 36-37 11.19451839567058 34.05490332998735 16.610657705232835 38.139920569109236 38-39 9.26125287322878 17.53469696528849 38.09246704879397 35.11158311268876 40-41 8.853374844514923 12.955998632458737 39.29280658747536 38.897819935550984 42-43 38.081556053420236 10.721871453926504 35.881899386074664 15.314673106578603 44-45 10.924088568186448 39.624370799266785 40.313218307195434 9.138322325351334 46-47 37.573831068695625 12.842241554889581 17.320114056271642 32.26381332014315 48-49 34.712463085002696 9.968140357002575 10.38493431676341 44.93446224123133 50-51 14.53261272794722 12.026739018163038 38.43697491216712 35.00367334172261 52-53 8.01594460123949 15.552532805726091 61.08920829817568 15.342314294858738 54-55 7.711164130466408 17.106258546946375 39.620733800808864 35.56184352177835 56-57 36.360462071172925 11.713271452265255 16.870835394419064 35.05543108214275 58-59 10.374249863611567 16.66412074922713 57.39952718676123 15.562102200400071 60-61 35.94928606239589 37.19094830407996 18.010285356823324 8.849480276700831 62-63 9.343313111916087 72.95712768588429 8.565006764718719 9.134552437480906 64-65 7.239450348805912 75.89492903854688 7.83085641126363 9.034764201383584 66-67 7.239861793053283 75.80578286961266 7.875613747954173 9.078741589379888 68-69 8.528158051704322 74.64902454282264 7.713458544888488 9.109358860584548 70-71 24.578644533835735 57.78807474922348 7.943377972401853 9.689902744538928 72-73 68.89079627865024 13.193479636593757 8.261018206681845 9.65470587807415 74-75 74.37477267767513 8.434567542009166 7.867898450571034 9.322761329744672 76 75.02255070270898 7.964035266389269 7.952396194023336 9.061017836878401 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 1.0 14 3.0 15 10.0 16 11.5 17 7.0 18 7.0 19 9.5 20 19.0 21 26.0 22 24.0 23 29.5 24 51.0 25 65.0 26 76.0 27 118.5 28 172.0 29 194.0 30 187.0 31 241.0 32 357.0 33 412.0 34 418.0 35 505.5 36 673.0 37 759.0 38 863.0 39 1113.0 40 1338.5 41 1599.5 42 1781.0 43 1940.5 44 2455.0 45 3314.5 46 3819.0 47 5318.0 48 10939.5 49 16765.5 50 18469.0 51 10575.0 52 2106.0 53 1211.5 54 892.0 55 840.0 56 731.0 57 646.0 58 618.0 59 561.5 60 459.0 61 381.0 62 349.0 63 350.5 64 307.0 65 246.5 66 199.5 67 168.0 68 155.0 69 133.0 70 116.5 71 109.0 72 104.5 73 93.0 74 89.5 75 93.0 76 69.0 77 43.5 78 33.5 79 25.0 80 24.5 81 18.0 82 11.0 83 10.0 84 11.0 85 9.0 86 6.5 87 7.0 88 4.0 89 3.0 90 5.0 91 3.5 92 2.0 93 3.0 94 4.0 95 8.0 96 12.0 97 19.5 98 39.0 99 291.5 100 532.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.011638225752484034 2 0.004364334657181512 3 0.0 4 0.0 5 0.0 6 0.0 7 0.004364334657181512 8 0.0 9 0.0 10-11 0.0 12-13 0.002182167328590756 14-15 0.0 16-17 0.002182167328590756 18-19 0.0 20-21 0.002182167328590756 22-23 0.0 24-25 7.273891095302521E-4 26-27 0.0029095564381210084 28-29 0.0014547782190605042 30-31 0.0014547782190605042 32-33 0.002182167328590756 34-35 0.0 36-37 0.0 38-39 0.0014547782190605042 40-41 0.00363694554765126 42-43 0.0014547782190605042 44-45 0.0014547782190605042 46-47 0.0014547782190605042 48-49 0.0 50-51 7.273891095302521E-4 52-53 0.0014547782190605042 54-55 0.0014547782190605042 56-57 0.00727389109530252 58-59 0.002182167328590756 60-61 7.273891095302521E-4 62-63 0.0 64-65 0.00654650198577227 66-67 0.002182167328590756 68-69 0.0029095564381210084 70-71 0.00363694554765126 72-73 7.273891095302521E-4 74-75 0.005819112876242017 76 0.00727389109530252 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 68739.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 35.08634108730124 #Duplication Level Percentage of deduplicated Percentage of total 1 81.01003399950245 28.42345684400413 2 8.636702877518866 6.0606060606060606 3 4.610664234181939 4.853140138785842 4 2.4214279791027447 3.398361919725338 5 1.144373496973215 2.007593942303496 6 0.6219421179202256 1.309300397154454 7 0.35243386682146116 0.865593040341 8 0.19902147773447218 0.5586348361192336 9 0.12438842358404512 0.39279011914633616 >10 0.6716974873538436 4.498174253335079 >50 0.08707189650883158 2.109428417637731 >100 0.08707189650883158 5.714368844469661 >500 0.01658512314453935 3.124863614541963 >1k 0.012438842358404511 8.408618106169714 >5k 0.0 0.0 >10k+ 0.004146280786134837 28.275069465659957 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCGT 19436 28.275069465659957 No Hit TCTTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCG 3121 4.540362821687833 No Hit CTAATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCGT 1567 2.27963746926781 No Hit CTCTTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCC 1092 1.5886178152140706 No Hit GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 557 0.810311468016701 No Hit CTTATACACATCTCCGCGCCCACGAGACCTTGGGTCATCTCGTATGCCGT 534 0.7768515689783093 No Hit CATATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCGT 530 0.7710324561020673 No Hit TCTTTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCC 527 0.7666681214448857 No Hit TCATATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCG 471 0.6852005411774975 No Hit ATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCGTCTT 418 0.6080972955672908 TruSeq Adapter, Index 7 (95% over 21bp) CTTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGACGT 322 0.4684385865374824 No Hit CTTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCACGTATGCCGT 308 0.4480716914706353 No Hit CTTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTAGTATGCCGT 230 0.334598990383916 No Hit CTTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTAAGCCGT 211 0.3069582042217664 No Hit CTTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCGA 196 0.28513653093585883 No Hit CTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCGTC 190 0.27640786162149583 No Hit TCTTATACACATCTCCGCGCCCACGAGACCTTGGGTCATCTCGTATGCCG 151 0.21967151107813612 No Hit CTTATACACATCTCCGAGCCCACGAGACCTAGGGTCATCTCGTATGCCGT 138 0.20075939423034958 No Hit CTTATACACATCTCCGAGCCCACGAGACCTTGGGTCATATCGTATGCCGT 138 0.20075939423034958 No Hit CTTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCAGT 134 0.19494028135410757 No Hit CTTATACACATCTCCGAGCCCACGAGACCATGGGTCATCTCGTATGCCGT 128 0.18621161203974454 No Hit CTTTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCG 118 0.1716638298491395 No Hit CAAATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCGT 116 0.1687542734110185 No Hit TCTTAATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCC 116 0.1687542734110185 No Hit CCTTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCG 114 0.1658447169728975 No Hit CTTATACACATCTCCGAGCCCACGAGACCTTGGGACATCTCGTATGCCGT 113 0.16438993875383698 No Hit CTTTACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCGTC 110 0.16002560409665545 No Hit CTTATACACATCTCCGAGCCCACGAGACATTGGGTCATCTCGTATGCCGT 105 0.15275171300135293 No Hit CTTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGAATGCCGT 101 0.14693260012511092 No Hit CTTAATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCG 96 0.1396587090298084 No Hit CTAATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTAAGCCGT 92 0.1338395961535664 No Hit CTTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATTCCGT 85 0.12365614862014288 No Hit CTAATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGACGT 85 0.12365614862014288 No Hit CTTAAACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCGT 84 0.12220137040108235 No Hit CTAATACACATCTCCGAGCCCACGAGACCTTGGGTCATCACGTATGCCGT 83 0.12074659218202184 No Hit CTAATACACATCTCCGCGCCCACGAGACCTTGGGTCATCTCGTATGCCGT 82 0.11929181396296135 No Hit TTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCGTC 74 0.10765358821047731 No Hit TATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCGTCT 73 0.10619880999141681 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.04073379013369412 0.0 2 0.0 0.0 0.0 0.04364334657181513 0.0 3 0.0 0.0 0.0 0.04364334657181513 0.0 4 0.0 0.0 0.0 0.046552903009936135 0.0 5 0.0 0.0 0.0 0.05528157232429916 0.0 6 0.0 0.0 0.0 0.05819112876242017 0.0 7 0.0 0.0 0.0 0.05819112876242017 0.0 8 0.0 0.0 0.0 0.05819112876242017 0.0 9 0.0 0.0 0.0 0.061100685200541176 0.0 10 0.0 0.0 0.0 0.06401024163866219 0.0 11 0.0 0.0 0.0 0.0669197980767832 0.0 12 0.0 0.0 0.0 0.07273891095302522 0.0 13 0.0 0.0 0.0 0.07273891095302522 0.0 14 0.0 0.0 0.0 0.07564846739114622 0.0 15 0.0 0.0 0.0 0.07564846739114622 0.0 16 0.0 0.0 0.0 0.07710324561020672 0.0 17 0.0 0.0 0.0 0.07710324561020672 0.0 18 0.0 0.0 0.0 0.08292235848644874 0.0 19 0.0 0.0 0.0 0.09165102780081176 0.0 20 0.0 0.0 0.0 0.09456058423893278 0.0 21 0.0 0.0 0.0 0.09892491889611428 0.0 22 0.0 0.0 0.0 0.10765358821047731 0.0 23 0.0 0.0 0.0 0.11929181396296135 0.0 24 0.0 0.0 0.0 0.12511092683920336 0.0 25 0.0 0.0 0.0 0.12802048327732438 0.0 26 0.0 0.0 0.0 0.12947526149638489 0.0 27 0.0 0.0 0.0 0.1367491525916874 0.0 28 0.0 0.0 0.0 0.15711604765853446 0.0 29 0.0 0.0 0.0 0.176028164506321 0.0 30 0.0 0.0 0.0 0.2094880635447126 0.0 31 0.0 0.0 0.0 0.22840018039249915 0.0 32 0.0 0.0 0.0 0.26476963586901175 0.0 33 0.0 0.0 0.0 0.2909556438121009 0.0 34 0.0 0.0 0.0 0.36951366764136806 0.0 35 0.0 0.0 0.0 0.4844411469471479 0.0 36 0.0 0.0 0.0 0.6037329609101093 0.0 37 0.0 0.0 0.0 0.8408618106169714 0.0 38 0.0 0.0 0.0 1.0838097732000755 0.0 39 0.0 0.0 0.0 1.3398507397547244 0.0 40 0.0 0.0 0.0 1.6031655974046757 0.0 41 0.0 0.0 0.0 1.8999403540930184 0.0 42 0.0 0.0 0.0 2.247632348448479 0.0 43 0.0 0.0 0.0 2.582231338832395 0.0 44 0.0 0.0 0.0 2.8528200875776486 0.0 45 0.0 0.0 0.0 3.2237885334380776 0.0 46 0.0 0.0 0.0 3.5889378664222638 0.0 47 0.0 0.0 0.0 3.929355969682422 0.0 48 0.0 0.0 0.0 4.214492500618281 0.0 49 0.0 0.0 0.0 4.547636712783136 0.0 50 0.0 0.0 0.0 4.800768122899664 0.0 51 0.0 0.0 0.0 5.196467798484121 0.0 52 0.0 0.0 0.0 5.529612010648977 0.0 53 0.0 0.0 0.0 5.820567654461078 0.0 54 0.0 0.0 0.0 6.085337290330089 0.0 55 0.0 0.0 0.0 6.387931159894674 0.0 56 0.0 0.0 0.0 6.725439706716711 0.0 57 0.0 0.0 0.0 6.980025895052299 0.0 58 0.0 0.0 0.0 7.237521639826008 0.0 59 0.0 0.0 0.0 7.445554925151661 0.0 60 0.0 0.0 0.0 7.707415004582551 0.0 61 0.0 0.0 0.0 7.928541293879748 0.0 62 0.0 0.0 0.0 8.154031917834127 0.0 63 0.0 0.0 0.0 8.402798993293473 0.0 64 0.0 0.0 0.0 8.587555827114157 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CACATAT 25 2.8126433E-6 70.00001 7 CTTTAAA 20 7.875757E-5 70.00001 57 ACATATC 20 7.875757E-5 70.00001 8 ATGCCAT 20 7.875757E-5 70.00001 44 ATATGCA 20 7.875757E-5 70.00001 52 CTTGAGT 20 7.875757E-5 70.00001 29 ATCCGAG 20 7.875757E-5 70.00001 12 GTAATCC 20 7.875757E-5 70.00001 42 ATATCCG 20 7.875757E-5 70.00001 10 CATATGC 25 2.8126433E-6 70.00001 51 CATATCC 20 7.875757E-5 70.00001 9 ATTCAGC 20 7.875757E-5 70.00001 51 TGAGTCA 20 7.875757E-5 70.00001 31 GTCATGA 15 0.002215987 70.0 34 TCGACCT 15 0.002215987 70.0 24 CAACCGA 15 0.002215987 70.0 10 GTCCGAA 15 0.002215987 70.0 43 TTCGACC 15 0.002215987 70.0 23 CGAAGGG 15 0.002215987 70.0 34 ATGTGTT 15 0.002215987 70.0 7 >>END_MODULE