FastQCFastQC Report
Wed 25 May 2016
SRR1780787_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780787_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences629617
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG553608.792646958388989No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTCCA200.006949210552.5026215
GGACCGC200.006951393652.4984550
GCCGTAT950.047.8895254
TCGCCGT900.046.66158752
TGGTCGC1000.045.50950249
CTCGGTG1100.041.37227244
CGGTGGT1200.040.8483546
TACATGC953.6379788E-1240.5315552
TCGGTGG1200.037.9306145
GCGAACT1051.4551915E-1136.66267428
GTACATG1151.8189894E-1236.532271
GTCGCCG1250.036.40181751
TGTGCGC400.00241801235.0017473
GATCTCG1201.8189894E-1234.99896641
TCTCGGT1800.033.06245843
AGATCTC1357.2759576E-1231.10772140
GTACTTA450.004310967431.10525126
CATCGTT802.4637775E-630.62896332
AAAGCGA1451.8189894E-1128.96236225
GCGGTCC500.00720617428.00139814
Produced by FastQC (version 0.11.5)