FastQCFastQC Report
Wed 25 May 2016
SRR1780787_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780787_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences629617
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCCGT318725.062125069685221No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCCG49860.7919100024300487No Hit
CTAATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCCGT29820.47362126499125656No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCC17240.273817257157923No Hit
CTTATACACATCTCCGCGCCCACGAGACTTATGAAAATCTCGTATGCCGT10290.16343269003219418No Hit
CATATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCCGT10280.16327386331690535No Hit
ATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCCGTCTT7720.12261422420296783RNA PCR Primer, Index 19 (95% over 23bp)
TCTTTATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCC7370.11705528916785919No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7280.11562584873025983No Hit
TCATATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCCG7110.1129257945703499No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAC800.065.6202348
AGGGGGG39450.062.17825370
ATCGTAT950.058.95245439
ACTCTCG1000.056.0003836
ATACCGT200.00695039752.50035544
GCCGTCT56800.052.3729646
CGTCTTC56500.052.2793948
TATGCCG59650.052.2133543
GTCTTCA2150.052.08923749
CGTATGC60550.052.0730141
CCGTCTT56350.052.05004547
TCGTATG61600.051.92392340
CTTGAAA59350.051.8958957
TCTCGTA61450.051.7741538
CTCGTAT61050.051.77350639
TGCCGTC59000.051.6105245
TCTTCTG56850.051.53069350
ATGCCGT59400.051.43974344
ATCTCGT61050.051.36808437
CTGCTTG57200.051.3418454