FastQCFastQC Report
Wed 25 May 2016
SRR1780786_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780786_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences755630
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG372274.926617524449797No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTCT252.3899305E-456.0027547
AGCCTTA952.8212162E-729.4751326
CTCTTCG808.667818E-526.24955419
TCCGTAT1001.3981224E-524.49958213
TACGGAC1752.0190782E-1023.99800338
GGTCTTC1202.16638E-623.334487
CAGTAGT901.9282433E-423.3329371
TGAGCGT1052.0396978E-523.33293527
TCTTCGT901.9291083E-423.33139220
CGCGGTA952.780504E-422.10488943
ACGGTTT952.780504E-422.10488912
GATCTTA800.00266657321.8746281
GATCCTA1454.6050627E-721.7237681
CCATAGG1454.6050627E-721.7237681
ACATGTT1304.3447853E-621.53666929
GACGGTT1003.9323617E-420.99964111
CCCAGTG1356.0244984E-620.73901420
AACGATT1709.3117706E-820.58788522
TGCCTGA850.003789954620.58788513
GGTCCTT1205.7151192E-520.416321
Produced by FastQC (version 0.11.5)