FastQCFastQC Report
Wed 25 May 2016
SRR1780786_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780786_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences755630
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTTAATGCATCTCGTATGCCGT208622.7608750314307264No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTTAATGCATCTCGTATGCCG53790.7118563318026019No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTTTAATGCATCTCGTATGCC19690.26057726665166814No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGAC900.058.33112745
AGGGGGG24800.056.4569570
TCGTATC200.006950340252.50148840
GTTGCGT200.006952159652.4980169
CGTATGA1700.049.41316641
GCCGTAT651.8553692E-1048.45970546
TGACGTC1450.048.2740445
AATCTCG800.048.12636636
CAACTCG1250.047.60135336
CTCGTAT44100.046.6679939
GCCGACT751.4551915E-1146.66490646
TATGCCG42250.046.5544543
CGTCTTC39850.046.54780248
TCGTATG44450.046.4580140
CGTATGC43200.046.34390641
CCGTCTT39500.046.25141547
TCTCGTA44300.046.14126238
GCCGTCT40600.046.0327446
ATCTCGT44300.045.90423637
AACTCGT1300.045.77052737
Produced by FastQC (version 0.11.5)