FastQCFastQC Report
Wed 25 May 2016
SRR1780785_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780785_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences235888
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3051112.934528250695246No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGTC207.922109E-569.9883356
CGCTACG207.922109E-569.98833549
CATCGTA150.002224175369.9883359
GTCGCCG252.3890937E-455.9906751
CGCGTCT252.3890937E-455.990677
GTGCGCG252.3890937E-455.990674
GTCCGTA200.006932326652.5246713
ACGTTCC200.00693814752.51352352
CGTTCCC200.00693814752.51352353
ACGTTGA200.00694979852.49125331
CCGGCGA200.00694979852.49125321
TGCGCGT200.00694979852.4912535
AGTTGCT200.00694979852.49125335
TGCTCGT200.00694979852.49125326
CCATTCG200.00694979852.49125367
GTCCTTA305.8623974E-446.6786881
CCCCAGT305.8623974E-446.6786881
CTTGCAC451.6819849E-646.65889427
GCCGGCG551.3083809E-744.53803620
CGATTTC802.910383E-1143.7612742
Produced by FastQC (version 0.11.5)