FastQCFastQC Report
Wed 25 May 2016
SRR1780785_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780785_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences235888
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCCGT157286.667571050668114No Hit
TCTTATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCCG44601.8907278030251644No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCC11580.4909109407854575No Hit
CTTATACACATCTCCGCGCCCACGAGACCTCATGGGATCTCGTATGCCGT6170.26156481041850366No Hit
TCTTTATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCC5830.24715119039544192No Hit
ATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCCGTCTT4240.1797463202875941RNA PCR Primer, Index 34 (95% over 23bp)
CTTATACACATCTCCGAGCCCACGAGACCTCATGGGATATCGTATGCCGT3870.16406091026249747No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGACGT3540.15007122024011393No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTAAGCCGT2910.12336363019738181No Hit
TCTTATACACATCTCCGCGCCCACGAGACCTCATGGGATCTCGTATGCCG2690.11403717018245949No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGTCA207.919854E-569.9923746
TAAGTGC150.00222366869.9923744
ACGTATG353.6998244E-969.9923740
ACGTAAT150.00222366869.9923747
AGGGGGG18000.061.269370
CGTCTAC308.343939E-658.3517148
CTTTATA1500.058.339342
TGCCGTA900.058.32697745
AACTCGT308.364948E-658.32697337
GCATGAA3900.056.532356
GTATTCT509.731593E-1056.0176549
AAGCCGT1000.055.99389344
TAAGACG509.767973E-1055.99389343
ACGACTT252.3884152E-455.99389347
TGGGAAC452.6935595E-854.4385133
CGTATGA1350.054.43850741
TGCATGA3100.053.05872755
CGAGTAT200.006948217352.49427841
TGACGAC200.006948217352.49427845
TCGGATC200.006948217352.49427833
Produced by FastQC (version 0.11.5)