FastQCFastQC Report
Wed 25 May 2016
SRR1780784_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780784_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38278
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCCGT1281433.476148179110716No Hit
TCTTATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCCG405710.598777365588589No Hit
CTCTTATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCC8122.1213229531323474No Hit
TCTTTATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCC5341.3950572130205339No Hit
ATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCCGTCTT3831.0005747426720308TruSeq Adapter, Index 10 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACACCGGCTAATATCGTATGCCGT2480.6478917393803229No Hit
CTTATACACATCTCCGCGCCCACGAGACACCGGCTAATCTCGTATGCCGT2230.5825800721040807No Hit
CTTATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGACGT2040.5329432049741366No Hit
CTTATACACATCTCCGAGCCCACGAGACACAGGCTAATCTCGTATGCCGT1990.5198808715188882No Hit
CATATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCCGT1770.462406604315795No Hit
CTTATACACATCTCCGAGCCCACGAGACACCGGCAAATCTCGTATGCCGT1590.4153822038769006No Hit
CTTAAACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCCGT1490.3892575369664037No Hit
CTATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCCGTC1460.3814201368932546No Hit
CTTATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCAGT1400.3657453367469565No Hit
TCTTATACACATCTCCGCGCCCACGAGACACCGGCTAATCTCGTATGCCG1330.34745806990960865No Hit
TCTTAATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCC1280.3343957364543602No Hit
CTTATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCCGA1170.30565860285281365No Hit
CTTATACACATCTCCGAGCCCACGAGACACCGGCTAAACTCGTATGCCGT1120.2925962693975652No Hit
CTTATACACATCTCCGAGCCCACGAGACAACGGCTAATCTCGTATGCCGT990.2586342024139192No Hit
CTTATACACATCTCCGAGCCCACGAGCCACCGGCTAATCTCGTATGCCGT900.23512200219447202No Hit
TCTTATACACATCTCCGAGCCCACGAGCCACCGGCTAATCTCGTATGCCG880.22989706881237265No Hit
TCTTATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCAG860.22467213543027326No Hit
CTTATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGAATGCCGT850.22205966873922356No Hit
CTTATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTAAGCCGT840.2194472020481739No Hit
TCTTATACACATCTCCGAGCCCACGAGACACCGGCTAATATCGTATGCCG780.20377240190187573No Hit
CCTTATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCCG760.19854746851977637No Hit
TCTTATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGACG750.1959350018287267No Hit
TATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCCGTCT740.193322535137677No Hit
CTTATACACATCTCCGAGCCCACGAGACACCGGCTATCTCGTATGCCGTC720.18809760175557763No Hit
CTTATACACATCTCCGAGCCCACGAGACACCGGATAATCTCGTATGCCGT710.18548513506452793No Hit
CTTTATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCCG700.18287266837347824No Hit
CTTATACACATCTCCGAGCCAACGAGACACCGGCTAATCTCGTATGCCGT680.17764773499137884No Hit
CTTATACACATCTCCGAGCCCACGAGACCCCGGCTAATCTCGTATGCCGT660.17242280160927947No Hit
CTTATACACATCTCCGAGACCACGAGACACCGGCTAATCTCGTATGCCGT660.17242280160927947No Hit
CTTATACACATCTCCGAGCCCACGAGACACCGGCTACTCTCGTATGCCGT660.17242280160927947No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG630.1645854015361304No Hit
CTTTACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCCGTC610.15936046815403102No Hit
TCTATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCCGT600.15674800146298135No Hit
CTAATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCCGT580.15152306808088198No Hit
ATTATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCCGT550.14368566800773291No Hit
TTATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCCGTC530.13846073462563352No Hit
CTTATACACATCTCCGAGCCCACGAGACACCGGCTAATCACGTATGCCGT490.12801086786143479No Hit
CTTAATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCCG480.1253984011703851No Hit
CTTATACACATCACCGAGCCCACGAGACACCGGCTAATCTCGTATGCCGT480.1253984011703851No Hit
CTTATACACATCTCCGAGCACACGAGACACCGGCTAATCTCGTATGCCGT470.12278593447933538No Hit
TCTTATACACATCTCCGAGCCAACGAGACACCGGCTAATCTCGTATGCCG460.12017346778828569No Hit
TCCTTATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCC430.11233606771513663No Hit
TCTTATACACATCTCCGAGCCCACGAGACACCGGCTACTCTCGTATGCCG390.10188620095093787No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACAGC207.831194E-570.051
TTATGCT252.7888036E-670.052
CAAGAAA207.831194E-570.057
ACTCGAA150.00220845370.038
CAGCATG252.7888036E-670.054
TACAGCA207.831194E-570.052
GCAAGAA150.00220845370.056
AACGAGC150.00220845370.013
TATGCTT207.831194E-570.053
CACAACT207.831194E-570.07
AAGAGAC207.831194E-570.022
AGGGCGG150.00220845370.070
TTACACA207.831194E-570.03
GAGCCCC150.00220845370.016
TCTACAG207.831194E-570.050
CGCTAAT150.00220845370.032
ACCGACT150.00220845370.029
TCAGCAT207.831194E-570.053
CCGTAAT150.00220845370.047
AGCGGGG207.831194E-570.070
Produced by FastQC (version 0.11.5)