FastQCFastQC Report
Wed 25 May 2016
SRR1780783_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780783_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences209119
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2502311.965914144577967No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAACCG200.00694947552.48977725
CGCTATT200.00694947552.4897778
AACGAGA200.00694947552.48977721
ACATGGA200.00694947552.48977731
CGTTTGT200.00694947552.48977722
TTCGGTA352.0839643E-549.9902726
CGGTAAG352.0839643E-549.9902728
GTGCAGC305.867026E-446.6687431
GCGAACT305.8739487E-446.6575828
GGTAAGC404.5871275E-543.7414829
CGTTCGG404.5871275E-543.7414824
TACCAGC503.4718414E-641.9918256
TCGTTCG459.186631E-538.88131723
TTACCAG556.6818884E-638.1743855
CCGGGGC400.00240873535.01830767
GTACATG400.002414397435.0015561
CTTTTTA708.651732E-735.0015561
GACCCCG400.002417232834.9931877
GTTCGGT501.7076643E-434.99318725
TACTCGC400.002417232834.99318720
Produced by FastQC (version 0.11.5)