FastQCFastQC Report
Wed 25 May 2016
SRR1780783_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780783_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences209119
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCGT125295.9913255132245276No Hit
TCTTATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCG34871.6674716309852284No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCC10800.5164523548792792No Hit
TCTTTATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCC4600.2199704474485819No Hit
CTTATACACATCTCCGCGCCCACGAGACCGGTTCCCATCTCGTATGCCGT4240.20275536895260593No Hit
ATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCGTCTT3580.17119439170998332RNA PCR Primer, Index 14 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGACGT2710.12959128534470804No Hit
CTTATACACATCTCCGAGCCCACGAGACCGGATCCCATCTCGTATGCCGT2430.11620177984783782No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGCG150.002220123870.017711
CGCTAAA150.002222231870.0009510
TGACGTA150.00222434169.98421545
AACGGTT650.059.23157527
AGGGGGG15100.059.1209170
ACGGTTC601.8189894E-1258.3341328
CTTTATA601.8189894E-1258.3201832
GTCTTCA1650.055.15226749
GGATCCC900.054.4451930
CCGTCAT2400.053.94616747
ATCGTAT850.053.51734539
ACCGGAT1050.053.3340627
GCCGTCA2450.052.84522246
GTTCCAC200.006930617652.52584564
GCGAGAC200.006937180652.5132811
TAACCGA200.006937180652.5132811
AACTTGG200.00694374852.50071728
TTGCGAG200.00694374852.5007179
GAACGGT608.367351E-1152.50071326
AGCCGAC200.006950319752.48816345