FastQCFastQC Report
Wed 25 May 2016
SRR1780782_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780782_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences187797
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1968510.482063078749928No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCGAG150.0022206970.0103911
CTGTTTA150.0022206970.0103944
CGCGCTT252.3872606E-455.9933978
GCGCTTC252.3872606E-455.9933979
TCGTTGT200.006946265752.4938134
TTCGGTC200.006946265752.4938128
TGTACGC200.006946265752.493814
ACGCGCT200.006946265752.493817
GATCCTA350.001252172740.0059361
CGAGTCT400.002415696234.99587222
CTAACCA400.002415696234.9958729
TAACCAT751.4844136E-632.66281510
TCATGTG450.00430185631.1074438
CATGTGA450.00430185631.1074439
CCATGAG500.007190037528.00415614
GCATGTA500.007190037528.0041562
CTATGGG500.00719939727.9966987
GAGTCTC500.00719939727.99669823
ACGACGA500.00719939727.99669870
GTATAAG959.3325325E-625.79331
Produced by FastQC (version 0.11.5)