FastQCFastQC Report
Wed 25 May 2016
SRR1780782_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780782_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences187797
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGTATGCCGT19631.0452776135934014No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGTATGCCG6060.32268886084442244No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTAGTATGCCGT5730.30511669515487466No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGTATTCCGT2700.14377226473266347No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGTATGACGT2630.14004483564700182No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTAGTATGCCG2170.11555030165551101No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACGCA150.002222467469.9962831
CGATTTT150.002222467469.9962822
AATGCCG252.8326358E-669.9962843
ATACCGT207.9134676E-569.9962744
GACGTAT352.9635703E-759.99680346
ATCGTAT252.3864984E-455.99701739
AGGGGGG5500.054.73893470
GCCGTAT850.053.5265646
ACAACGC200.006944489752.49720427
AGCAATT200.006944489752.4972042
TAGACTT200.006944489752.49720425
ATATCGT200.006944489752.49720437
AATCTCG200.006944489752.49720436
GTCCGGT200.006944489752.49720428
GCGATTT200.006944489752.49720421
ATAACGA200.006944489752.49720444
GTCCAGA200.006944489752.4972042
GACGAAT200.006944489752.49720446
AATACGT200.006944489752.49720444
GCATATC200.006944489752.49720435