FastQCFastQC Report
Wed 25 May 2016
SRR1780781_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780781_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences98660
Sequences flagged as poor quality0
Sequence length76
%GC61

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2383124.154672613014394No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTCCC150.002221350669.9802326
GCGACAG308.312578E-658.3464335
CGACAGC308.312578E-658.3464336
GATAGTC252.3776555E-456.01257752
CGATTTC200.006927143352.5117942
TTCGTGA200.00694105552.48517222
CTTATCT200.00694105552.4851724
CTACAGG200.00694105552.48517226
CGGCTGG200.00694105552.4851729
GCTACAG200.00694105552.48517225
GTCAGTA305.846674E-446.67714756
CAGTAGG305.846674E-446.67714758
CCTTAAC305.861316E-446.6534841
AAACGTA305.861316E-446.65348415
TACTAGA305.861316E-446.6534841
GCTTAGG404.558119E-543.75982766
TTTTTCG602.573397E-740.821819
TTAAGGC350.001248814740.00898430
TCGTTCG350.001251934839.98870523
AAAACGT350.001251934839.98870514
Produced by FastQC (version 0.11.5)