FastQCFastQC Report
Wed 25 May 2016
SRR1780781_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780781_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences98660
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCCGT1184912.00993310358808No Hit
TCTTATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCCG36283.6772754915872694No Hit
CTTATACACATCTCCGAGCCCACGAGACGCCGCTCCATCTCGTATGCCGT8710.8828299209406041No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCC8700.8818163389418204No Hit
TCTTTATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCC5100.5169268193796878No Hit
ATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCCGTCTT4150.4206365294952361Illumina PCR Primer Index 11 (95% over 22bp)
TCTTATACACATCTCCGAGCCCACGAGACGCCGCTCCATCTCGTATGCCG2990.30306101763632676No Hit
CTTATACACATCTCCGCGCCCACGAGACGACGCTCCATCTCGTATGCCGT2430.2463004257044395No Hit
CTTATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGACGT1960.19866207176160552No Hit
CTTATACACATCTCCGAGCCCACGAGACGACGCTCAATCTCGTATGCCGT1650.16724102979931077No Hit
CTTATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCCGA1510.15305088181633894No Hit
CATATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCCGT1490.15102371781877155No Hit
CTTAAACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCCGT1390.1408878978309345No Hit
CTTATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTAAGCCGT1310.13277924184066492No Hit
CTTATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCAGT1300.1317656598418812No Hit
CTTATACACATCTCCGAGCCCACGAGACGACGCTCCATATCGTATGCCGT1180.11960267585647678No Hit
CTATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCCGTC1150.11656192986012569No Hit
CTTATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGAATGCCGT1130.11453476586255829No Hit
CTTATACACATCTCCGAGCCCACGAGACGAAGCTCCATCTCGTATGCCGT1120.11352118386377458No Hit
TCTTATACACATCTCCGCGCCCACGAGACGACGCTCCATCTCGTATGCCG1090.11048043786742348No Hit
TCTTAATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCC1030.10439894587472127No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCTTTA150.002217458370.0111655
ACGTTCC150.002217458370.0111632
TGACGAC301.0134863E-770.0111645
ATCGTAA150.002217458370.0111639
ATATAAG150.002217458370.0111659
TCGTATT150.002217458370.0111640
ATGACGA301.0134863E-770.0111644
CACGTAT401.3278623E-1070.0111639
AGCTAGA252.8190934E-670.01115455
ATGGGTG150.002221924569.975685
TTGGACC150.002221924569.975688
GGGTATC150.002221924569.975689
AGGGGGG13750.062.9146570
ATATCGT850.057.65624637
ATCACGT509.658834E-1056.00892337
TATCTTA252.3784215E-456.00892326
CAGCTAG252.3784215E-456.00892354
ACGTATG509.658834E-1056.00892340
ATCGTAT850.053.5379439
CATATCG800.052.5083736
Produced by FastQC (version 0.11.5)