FastQCFastQC Report
Wed 25 May 2016
SRR1780780_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780780_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences120305
Sequences flagged as poor quality0
Sequence length76
%GC60

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2569421.357383317401606No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTTGGC207.899842E-570.056
TATCCGG150.002220052570.053
TTGGCGG252.382412E-455.99999658
AATTTCC200.006936991652.546
GATAACT200.006936991652.536
AGATAAC200.006936991652.535
AACTTGG200.006936991652.557
CGGCAGT200.006936991652.56
CCGGAAT200.006936991652.556
CCCAGAG200.006936991652.567
GAATTTC200.006936991652.545
GTTGGCG200.006936991652.557
CTAATGT200.006936991652.51
GACTATA200.006936991652.510
TGGTCGC305.858323E-446.66666455
CGCTGGC404.5701978E-543.7516
CTGGCGG556.6493594E-638.18181618
CGACGAG400.002410862135.022
CTATCCG400.002410862135.052
TGGCGCT501.7014137E-435.013
Produced by FastQC (version 0.11.5)