FastQCFastQC Report
Wed 25 May 2016
SRR1780780_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780780_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences120305
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCCGT1475312.262998212875607No Hit
TCTTATACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCCG37993.1578072399318398No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCC12421.0323760442209384No Hit
TCTTTATACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCC4640.3856863804496904No Hit
ATACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCCGTCTT3870.3216823905905823TruSeq Adapter, Index 9 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACGGAGAAAGATCTCGTATGCCGT3250.27014671044428745No Hit
CTTATACACATCTCCGAGCCCACGAGACGGAGTAAGCTCTCGTATGCCGT3050.2535222974938698No Hit
CTTATACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGACGT2530.21029882382278378No Hit
CTTATACACATCTCCGCGCCCACGAGACGGAGTAAGATCTCGTATGCCGT2300.1911807489298034No Hit
CATATACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCCGT2150.17871243921699015No Hit
CTTAAACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCCGT1820.15128215784880095No Hit
CTTATACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCAGT1740.14463239266863387No Hit
CTTATACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCCGA1560.12967042101325796No Hit
CTTATACACATCTCCGAGCCCACGAGACGGAGTAAGATATCGTATGCCGT1340.11138356676779851No Hit
CTTATACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTAAGCCGT1280.1063962428826732No Hit
CTTATACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGAATGCCGT1220.1014089189975479No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCTTC150.002220104769.9995938
GACGTCA150.002220104769.9995946
TTAGTGC150.002220104769.9995956
CCTGGAC150.002220104769.999599
TAGTGCC150.002220104769.9995957
AGGGGGG17000.064.2349270
CGTCTAC800.061.24963848
GATCTAG401.05646905E-861.24963836
ACGCAGT352.953584E-759.99964527
GACCACG850.057.6467218
GTCTACT700.054.99967649
AAAGATC1600.054.68717633
ACTGCTT653.6379788E-1253.84583353
GCCGTCA1400.052.4996946
GATACAA200.006937155552.49968716
CACGAGG200.006937155552.49968714
CGCGAAA200.006937155552.49968744
TTCCCTA200.006937155552.49968731
AGTGCCT200.006937155552.49968758
TCCACGA200.006937155552.49968712
Produced by FastQC (version 0.11.5)