FastQCFastQC Report
Wed 25 May 2016
SRR1780779_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780779_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences273360
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2952210.799678080187299No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCCGA200.006939579352.51303562
TTTCGTC200.006944603352.5034330
CAGGGTA200.0069496352.4938289
GACCTAT200.0069496352.4938281
CTTGGTT350.001253687340.00261312
ACGATTC350.001253687340.00261321
CGACCTT400.002417614234.9958845
GCTTGGT552.9863857E-431.8202611
CATCTTG450.00430525131.10745442
ATCTTGC450.00430525131.10745443
CCCCTCT500.00720504527.9967084
CCATAGA500.00720504527.9967082
GACATAT500.00720504527.9967081
TGCTTGG657.949198E-426.91991210
ATAGGAC657.949198E-426.9199128
GCCATAG657.949198E-426.9199121
TCCACAT657.949198E-426.9199122
GGGTACA700.001222391825.00620862
CGTGTCG700.001225003224.9970642
TACGTGT700.001225003224.9970640
Produced by FastQC (version 0.11.5)