FastQCFastQC Report
Wed 25 May 2016
SRR1780779_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780779_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences273360
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCATCCTGTATCTCGTATGCCGT137005.011706175007316No Hit
TCTTATACACATCTCCGAGCCCACGAGACCATCCTGTATCTCGTATGCCG36601.3388937664618086No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCATCCTGTATCTCGTATGCC10430.38154814164471756No Hit
CTTATACACATCTCCGAGCCCACGAGACCATCCTGTATCTAGTATGCCGT9060.33143107989464443No Hit
CTTATACACATCTCCGAGCCCACGAGACCATCCTGTATCTCGTATGACGT5120.18729880011706176No Hit
CTTATACACATCTCCGAGCCCACGAGACCATCCTGTATCTCGTAAGCCGT4840.17705589698565993No Hit
TCTTTATACACATCTCCGAGCCCACGAGACCATCCTGTATCTCGTATGCC3850.14083991805677493No Hit
CTTATACACATCTCCGCGCCCACGAGACCATCCTGTATCTCGTATGCCGT3180.11633011413520632No Hit
CTTATACACATCTCCGAGCCCACGAGACCATCCTGTATCTCGTATTCCGT3000.10974539069359088No Hit
CTTATACACATCTCCGAGCCCACGAGACAATCCTGTATCTCGTATGCCGT2890.10572139303482588No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGTAT207.921414E-569.993646
CCGTCAA207.921414E-569.993647
TAACTCG600.064.1725436
AACTCGT550.063.64218537
AGGGGGG13400.061.39744670
AATCTCG800.061.25560836
GCCGTAA1500.055.9948846
GAATCTC1050.053.3382135
TTCCGTA200.0069489152.49519745
TCCGTCA407.4678974E-752.49519746
ATAACTC200.0069489152.49519743
TAATCCG200.0069489152.49519743
GCCGTCA5900.052.1986246
CCGTAAT1700.051.4658847
CGTCATA1500.051.3286448
TGCCGTA2600.051.1491745
GCATGAA7200.050.07402856
CGACTTC352.0843898E-549.9954348
GCCGTAT1400.049.9954346
CGTATGC25650.049.80962441