FastQCFastQC Report
Wed 25 May 2016
SRR1780778_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780778_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences212712
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2347611.036518861183197No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTCAC252.3848884E-456.00809513
CGTCACC252.3848884E-456.00809514
CCGTCAT200.00694677952.49523521
TCTGTGA200.006953242752.48289534
ACTCCCG305.877975E-446.65146650
ACTGCCC350.001252571940.0057836
CTCCCGA350.001254021839.99637251
AGGTGCA350.001254021839.99637251
TCGGGCC400.002407739935.02152641
TAACGAC400.002410521535.0132954
TACATGG400.002413305535.00505417
AGCTCGT552.9827846E-431.82277911
TAGCTCG552.9869043E-431.81529610
TGCGAAG450.00429265831.12292738
CCCGATT450.00429265831.12292753
CCCGTCA450.00429760431.11560612
CGTACAC450.00429760431.1156061
ACACTTC450.00429760431.11560643
CGAGAAT450.00429760431.11560614
TTGATAA450.004302554331.108299