FastQCFastQC Report
Wed 25 May 2016
SRR1780778_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780778_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences212712
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGCCGT127625.999661514159991No Hit
TCTTATACACATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGCCG38161.7939749520478394No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGCC10050.47246981834593255No Hit
TCTTTATACACATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGCC3830.1800556621159126No Hit
ATACACATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGCCGTCTT3350.15748993944864417No Hit
CTTATACACATCTCCGAGCCCACGAGACGCGATAAAATCTCGTATGCCGT3100.14573695889277521No Hit
CTTATACACATCTCCGCGCCCACGAGACGCGTTAAAATCTCGTATGCCGT2340.11000789800293355No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGAGAT150.002221457470.0075227
AGGGGGG15200.061.96201370
ACGAGTT352.9621697E-760.0064527
GAGACGA601.8189894E-1258.33960324
ACGTATG553.6379788E-1157.26542340
CGAGTTA452.6922862E-854.43749628
CGAATGC900.054.43749641
ACGCGAA200.006941336752.50564627
TTATCGG200.006941336752.50564625
AAAACGG407.449744E-752.50564667
AAACACG200.00694779552.49329836
CATCGTT200.00694779552.49329832
TGCCGTA200.00694779552.49329845
CAGACTA200.00694779552.4932981
AATCACG552.2737368E-950.90259636
TCGAATG900.050.54910340
ATCGTAT707.2759576E-1249.99361839
GCCGACA352.0832596E-549.99361846
CGTCTAC651.8371793E-1048.46674748
GCCGTCT26000.047.51316546