FastQCFastQC Report
Wed 25 May 2016
SRR1780777_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780777_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences113129
Sequences flagged as poor quality0
Sequence length76
%GC62

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2751124.31825615005878No Hit
CTGTCTCTTATACACATCTGACGCTGCCGACGACCAAGCCCGTGTAGATC1230.1087254373326026No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGCGA150.002220485669.9938110
CCCGTGC150.002220485669.993818
TAGCCCG308.335912E-658.3281755
TGTGCGC200.006926218552.5185783
TCTAGCA200.006926218552.5185783
GATATTA200.006926218552.5185781
TAGTACT200.006926218552.51857849
GCGAGAA200.006938348552.49535813
TACTTGG200.006938348552.4953587
CGACGTG200.006938348552.49535839
CCGTGCG200.006938348552.4953589
TAGCCTT200.006938348552.4953585
CTGGTGT200.006938348552.4953589
CCACTTC200.006938348552.49535838
TGCGCGT200.006938348552.4953585
CCATCTA200.006938348552.49535828
TACGATA200.006938348552.49535854
TTCCACC200.006938348552.49535839
CCATATG200.006938348552.49535827
CTGTTAC200.006938348552.4953589
Produced by FastQC (version 0.11.5)