##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780777_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 113129 Sequences flagged as poor quality 0 Sequence length 76 %GC 62 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 27.138160860610455 32.0 27.0 32.0 13.0 32.0 2 28.202715484093382 32.0 27.0 32.0 13.0 32.0 3 27.955776149351625 32.0 27.0 32.0 13.0 32.0 4 27.159932466476324 32.0 27.0 32.0 13.0 32.0 5 27.73912966613335 32.0 27.0 32.0 13.0 32.0 6 31.405713831113154 37.0 32.0 37.0 13.0 37.0 7 31.06883292524463 37.0 32.0 37.0 13.0 37.0 8 31.216911667211768 37.0 32.0 37.0 13.0 37.0 9 31.272016901059853 37.0 32.0 37.0 13.0 37.0 10-11 31.025254355647093 37.0 29.5 37.0 13.0 37.0 12-13 30.991447816209813 37.0 27.0 37.0 13.0 37.0 14-15 31.654310565814246 37.0 32.0 37.0 13.0 37.0 16-17 31.67928647826817 37.0 32.0 37.0 13.0 37.0 18-19 31.170119951559723 37.0 27.0 37.0 13.0 37.0 20-21 31.554517409329172 37.0 29.5 37.0 13.0 37.0 22-23 31.521250077345332 37.0 29.5 37.0 13.0 37.0 24-25 31.05449531066305 37.0 27.0 37.0 13.0 37.0 26-27 31.48518505423013 37.0 29.5 37.0 13.0 37.0 28-29 31.319798636954275 37.0 32.0 37.0 13.0 37.0 30-31 31.149917350988694 37.0 32.0 37.0 13.0 37.0 32-33 31.339691856199558 37.0 32.0 37.0 13.0 37.0 34-35 31.275252145780478 37.0 32.0 37.0 13.0 37.0 36-37 31.780657479514538 37.0 32.0 37.0 13.0 37.0 38-39 31.852999672939745 37.0 32.0 37.0 13.0 37.0 40-41 31.348442927984866 37.0 29.5 37.0 13.0 37.0 42-43 31.188081747385727 37.0 32.0 37.0 13.0 37.0 44-45 30.87762200673567 37.0 27.0 37.0 13.0 37.0 46-47 31.146120800148502 37.0 29.5 37.0 13.0 37.0 48-49 31.643278911684888 37.0 32.0 37.0 13.0 37.0 50-51 31.91139318830715 37.0 32.0 37.0 13.0 37.0 52-53 30.957667795171886 37.0 29.5 37.0 13.0 37.0 54-55 31.061253082763926 37.0 29.5 37.0 13.0 37.0 56-57 30.822180873162495 37.0 27.0 37.0 13.0 37.0 58-59 30.432364822459316 37.0 27.0 37.0 13.0 37.0 60-61 30.274819011924443 37.0 27.0 37.0 13.0 37.0 62-63 30.048130894819188 37.0 27.0 37.0 10.5 37.0 64-65 29.995531649709626 37.0 24.5 37.0 10.5 37.0 66-67 29.98498174650178 37.0 27.0 37.0 10.5 37.0 68-69 30.141422623730435 37.0 27.0 37.0 13.0 37.0 70-71 30.211338383615164 37.0 27.0 37.0 13.0 37.0 72-73 30.53450043755359 37.0 27.0 37.0 13.0 37.0 74-75 30.823343263000645 37.0 27.0 37.0 13.0 37.0 76 31.01487682203502 37.0 27.0 37.0 13.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 11101 1 1.166600870578641 11101 2 -0.24618352829489965 11101 3 0.1967730967765604 11101 4 0.05460951567868122 11101 5 -1.319026119781931 11101 6 -0.2539371883682122 11101 7 0.33645276017000114 11101 8 0.9264143881731357 11101 9 1.1573786016970473 11101 10-11 0.25820168468586147 11101 12-13 0.9199954495015099 11101 14-15 0.4729870039964936 11101 16-17 0.357611085563331 11101 18-19 0.2625615398220589 11101 20-21 -0.6193400188591944 11101 22-23 -0.8684289695733654 11101 24-25 0.4250959177856295 11101 26-27 -1.0764320093856483 11101 28-29 0.24680275305661326 11101 30-31 0.4251701641451682 11101 32-33 -0.26724577404538863 11101 34-35 -0.7746604754078241 11101 36-37 -1.899021562589251 11101 38-39 -0.465107073904484 11101 40-41 -1.2439784867987598 11101 42-43 0.0909671335820228 11101 44-45 -1.0402428656192946 11101 46-47 0.17697050393815772 11101 48-49 -0.02200175630669321 11101 50-51 -0.1596278244435183 11101 52-53 -0.8235967599385354 11101 54-55 -0.4879420406115891 11101 56-57 -0.2935647307027658 11101 58-59 -0.20645517025003102 11101 60-61 -1.0697643623127782 11101 62-63 -0.21213902235594162 11101 64-65 -1.0994700942562154 11101 66-67 -0.20718252196286358 11101 68-69 -1.289219917967987 11101 70-71 -1.0826097055078172 11101 72-73 -0.9062416882325621 11101 74-75 -0.8046050495369172 11101 76 0.9743611886632983 11102 1 -0.7281359715266227 11102 2 -0.604579518269837 11102 3 -0.01876575034123462 11102 4 -0.84179817020269 11102 5 0.2139730447961803 11102 6 0.6006994031104895 11102 7 -0.8799215088358459 11102 8 -1.182190457273812 11102 9 0.07300099100364932 11102 10-11 -0.7844048316048635 11102 12-13 -1.5286261043832 11102 14-15 -0.9798116593284902 11102 16-17 -0.9076353638936432 11102 18-19 -1.2775387108045066 11102 20-21 0.24030910394782623 11102 22-23 -0.4323387440094528 11102 24-25 -0.7662156945786158 11102 26-27 -0.16498672951931326 11102 28-29 -1.8709917331589274 11102 30-31 -1.1767513061973567 11102 32-33 0.2895629143422447 11102 34-35 -0.36488603931759656 11102 36-37 0.3967177857816786 11102 38-39 -1.265441242659712 11102 40-41 -2.3116476597310935 11102 42-43 -0.2160504102776244 11102 44-45 -0.27959123654661155 11102 46-47 0.08674494002838173 11102 48-49 -1.540381037843872 11102 50-51 -0.09279407339924006 11102 52-53 -1.1707145544247517 11102 54-55 -1.8764132185564542 11102 56-57 -1.184959885255818 11102 58-59 0.36330255740243445 11102 60-61 -1.066840385704591 11102 62-63 -2.035029582088608 11102 64-65 -2.4774984986004114 11102 66-67 -2.1123621376687964 11102 68-69 0.22205827752073404 11102 70-71 0.06901936717389034 11102 72-73 0.026506849779135422 11102 74-75 -0.11496428094878297 11102 76 -1.0122720611278453 11103 1 -0.23607247946312881 11103 2 -0.4617223754126947 11103 3 -0.06638479796028207 11103 4 -0.84179817020269 11103 5 -0.4050745742514401 11103 6 0.06101686342794821 11103 7 -0.8164294453437826 11103 8 0.5479682728849191 11103 9 0.35871527671793757 11103 10-11 -2.1812302284302625 11103 12-13 -1.131800707557808 11103 14-15 0.21066453114770312 11103 16-17 -0.20128615754443757 11103 18-19 -0.031506964772759716 11103 20-21 -0.4263575627188452 11103 22-23 -2.1148784265491365 11103 24-25 -0.46462839299131176 11103 26-27 -0.6887962533288388 11103 28-29 -0.4662298283970223 11103 30-31 -1.4227830522291036 11103 32-33 -2.139008514229186 11103 34-35 -1.9998066742382328 11103 36-37 0.14274953181342553 11103 38-39 0.4488444716260034 11103 40-41 -1.9545048025882323 11103 42-43 -2.390653584880802 11103 44-45 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0.27718506551435595 11104 24-25 -2.008676012038933 11104 26-27 -1.0729232374558215 11104 28-29 -1.7317060188732114 11104 30-31 0.10499472554867495 11104 32-33 -1.7124212126418819 11104 34-35 -0.7470288964604528 11104 36-37 -0.06042507136117692 11104 38-39 0.5647174874990171 11104 40-41 -0.9295048025882338 11104 42-43 -1.432717076944293 11104 44-45 0.6799325729771972 11104 46-47 0.36293541621885694 11104 48-49 -1.4149842124470453 11104 50-51 -0.6578734384786067 11104 52-53 -1.7433336020438013 11104 54-55 0.1331105909673589 11104 56-57 -1.6365471868431172 11104 58-59 -1.0656656965658193 11104 60-61 0.044270725406523326 11104 62-63 -0.8858232328822595 11104 64-65 -2.085435006536919 11104 66-67 0.13009817979152416 11104 68-69 -0.6291321986697405 11104 70-71 -1.1738377756832534 11104 72-73 -1.3957153724430889 11104 74-75 -1.0883769793614775 11104 76 -0.7686212674770516 11105 1 0.09726085387020333 11105 2 0.062087148396827274 11105 3 -0.9076546392301239 11105 4 -0.01640134480586397 11105 5 -1.6114237806006457 11105 6 -1.3834275810164982 11105 7 0.6438880149736796 11105 8 0.24638097129761505 11105 9 1.0412549592576212 11105 10-11 -0.26059530779534157 11105 12-13 0.4316913559342588 11105 14-15 -0.9956846752015025 11105 16-17 -1.3520798083380932 11105 18-19 -0.3410307742965699 11105 20-21 -1.0930242293855095 11105 22-23 -0.9720212836919941 11105 24-25 -0.9090728374357582 11105 26-27 -0.8792724438050286 11105 28-29 -1.0455949077621014 11105 30-31 -0.7481798776259296 11105 32-33 -0.8374212126418819 11105 34-35 -2.063298737730296 11105 36-37 -1.1032822142183214 11105 38-39 -1.5035364807549456 11105 40-41 -2.43863178671522 11105 42-43 -0.1843043785315963 11105 44-45 -0.6287975857529595 11105 46-47 -0.4211915679081244 11105 48-49 -1.7070477045105363 11105 50-51 -0.03723851784368648 11105 52-53 -2.4564288401390364 11105 54-55 -0.44784178998502355 11105 56-57 -0.43892813922407115 11105 58-59 -1.8906656965658186 11105 60-61 -0.4398562587204644 11105 62-63 -1.0747121217711495 11105 64-65 -0.858450879552791 11105 66-67 0.34001881471215967 11105 68-69 -2.0636560081935507 11105 70-71 -0.8278060296515086 11105 72-73 -1.6480963248240421 11105 74-75 -0.16258332856782687 11105 76 -2.1710022198580035 11106 1 0.08369198546262524 11106 2 0.6143144909467502 11106 3 0.7400668149429421 11106 4 -1.432940003280006 11106 5 0.5516586566753396 11106 6 -0.027564805798885317 11106 7 0.6075336882967726 11106 8 0.6086441561414482 11106 9 -2.2769734073578434 11106 10-11 -0.8128226503452645 11106 12-13 -0.13461688779334224 11106 14-15 -0.2357000361846069 11106 16-17 1.6451040114263797 11106 18-19 0.4544121340495657 11106 20-21 -0.5930242293855095 11106 22-23 -0.5771160097544623 11106 24-25 -0.35786956042602824 11106 26-27 0.20342084856568476 11106 28-29 -1.0249855887656878 11106 30-31 -1.2982310809029407 11106 32-33 0.4851594325194064 11106 34-35 1.2941001357976099 11106 36-37 -0.47117775953322294 11106 38-39 -0.3040997168020567 11106 40-41 -1.1910639423731837 11106 42-43 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11107 22-23 -0.367438590399626 11107 24-25 -0.4062566572002204 11107 26-27 0.5501950421140691 11107 28-29 -0.7427275242495597 11107 30-31 0.3953173061938351 11107 32-33 -1.9503244384483303 11107 34-35 0.13280981321696217 11107 36-37 -0.8985971143719311 11107 38-39 -0.9734545555117293 11107 40-41 0.5428070253687522 11107 42-43 -1.170351485546444 11107 44-45 1.1724056912567669 11107 46-47 -0.57147318593168 11107 48-49 -0.32251109416747425 11107 50-51 -0.9283035460054876 11107 52-53 -0.28822607516208265 11107 54-55 0.40085252645122793 11107 56-57 -0.7714934234022586 11107 58-59 -0.4471173094690428 11107 60-61 -0.3939013176042323 11107 62-63 -0.2772210823446244 11107 64-65 -0.017693071053045628 11107 66-67 -1.2841491320364327 11107 68-69 0.49586780133025954 11107 70-71 -0.4743754100918558 11107 72-73 1.0757900039009947 11107 74-75 1.5817843109610976 11107 76 -0.1756105147888789 11108 1 0.5077614643708159 11108 2 0.48553037184005277 11108 3 -2.3125386441141274 11108 4 1.532072403667886 11108 5 -1.122534891711755 11108 6 0.5376957401068232 11108 7 -1.1175283464426826 11108 8 -1.2139364890198436 11108 9 -3.2901247721221125 11108 10-11 -0.4225000697001029 11108 12-13 -0.33412060987770786 11108 14-15 -0.3839630634798894 11108 16-17 -0.8390150952733784 11108 18-19 -1.2815680148338089 11108 20-21 -2.1468703832316614 11108 22-23 -0.11768672935744107 11108 24-25 0.09324706488414236 11108 26-27 -0.5062565707891551 11108 28-29 0.29201192984473323 11108 30-31 -2.414236043682095 11108 32-33 -1.2143442895649592 11108 34-35 -3.014336588768149 11108 36-37 -1.316835327771436 11108 38-39 -0.5993850766035465 11108 40-41 -2.0878381359215687 11108 42-43 -0.5878452820725002 11108 44-45 -1.857246914202289 11108 46-47 -0.6960389427555 11108 48-49 -0.17908677654961025 11108 50-51 0.7780239974188312 11108 52-53 -0.5234618071720085 11108 54-55 -0.520735562878798 11108 56-57 0.1878117875158587 11108 58-59 -1.9752810811812047 11108 60-61 -0.9582933771575846 11108 62-63 -2.419156566215591 11108 64-65 -0.5162042373061482 11108 66-67 -0.7077223330289897 11108 68-69 -0.7079783525158945 11108 70-71 -0.7328121346576104 11108 72-73 -1.144433321161035 11108 74-75 -1.9749154408999416 11108 76 -1.4699033187591048 11109 1 0.10270979961765647 11109 2 -0.6386946568625902 11109 3 -1.379817633781176 11109 4 0.8985477193969338 11109 5 -1.8120871305177282 11109 6 -0.9096062116727417 11109 7 -0.3857258206103076 11109 8 -1.4676678323034267 11109 9 0.14454801777455728 11109 10-11 -0.977723950297122 11109 12-13 -1.4273467866859733 11109 14-15 -1.1669711002192713 11109 16-17 -0.59538708149611 11109 18-19 -0.3525209425031548 11109 20-21 -0.8169048264004353 11109 22-23 0.05131441874818421 11109 24-25 -1.3597954150240064 11109 26-27 -0.1107341827294519 11109 28-29 -0.8830741780771945 11109 30-31 -0.756945572958788 11109 32-33 0.3566086381043867 11109 34-35 -1.513073672579857 11109 36-37 0.01221174455922025 11109 38-39 -1.988018830908942 11109 40-41 -1.5714202254738083 11109 42-43 -0.7359509077900626 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21612 18-19 -0.3453042785700724 21612 20-21 0.21466807830679713 21612 22-23 -0.8077966194673287 21612 24-25 -0.4847749131378336 21612 26-27 -0.5337290982616807 21612 28-29 -0.14754850971570477 21612 30-31 0.2341156046695545 21612 32-33 0.8109304357097677 21612 34-35 -1.6451058195373776 21612 36-37 -1.0410111519472593 21612 38-39 -0.06641804363651005 21612 40-41 -1.6065194546028856 21612 42-43 -0.2691639633911791 21612 44-45 -0.28142273837811516 21612 46-47 -0.8235114702280271 21612 48-49 -0.31205380951664097 21612 50-51 -0.5824155630207315 21612 52-53 0.25236236865217165 21612 54-55 -1.8306256727689068 21612 56-57 -1.4902101905061222 21612 58-59 -1.3357206416207639 21612 60-61 -1.1846670035312066 21612 62-63 -1.3323433794024027 21612 64-65 -0.8920284131303262 21612 66-67 -0.560469585776243 21612 68-69 -1.6881981327356748 21612 70-71 -2.020724222569701 21612 72-73 -1.5015761783038961 21612 74-75 -1.9012890672735665 21612 76 -0.7204527693085545 >>END_MODULE >>Per sequence quality scores pass #Quality Count 10 3.0 11 14.0 12 55.0 13 166.0 14 309.0 15 529.0 16 775.0 17 1003.0 18 1289.0 19 1524.0 20 1865.0 21 2190.0 22 2576.0 23 3026.0 24 3298.0 25 3518.0 26 3672.0 27 4073.0 28 4368.0 29 4877.0 30 5433.0 31 6560.0 32 7922.0 33 10504.0 34 14650.0 35 16992.0 36 11938.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 59.1310497237569 15.93900552486188 7.602209944751381 17.327734806629834 2 44.20851639366 17.137982549967735 23.897881067510585 14.755619988861682 3 43.28135221626974 18.492193992114426 21.506745168761825 16.71970862285401 4 40.38398642257953 15.743973693747845 25.33656268507633 18.535477198596293 5 39.70104660207906 25.41457464111449 25.632027437946398 9.252351318860052 6 49.75956438724277 27.962131390990734 13.46439431440492 8.813909907361573 7 48.847303007266234 22.945211534041686 15.511023106978058 12.696462351714018 8 47.904143978502226 25.5427480376211 14.00095467081536 12.552153313061313 9 46.954723052171914 14.298216148365539 14.209819139720311 24.537241659742236 10-11 48.27825487396852 19.491896240049854 18.752458530936604 13.477390355045015 12-13 47.40268637321936 18.70030452634882 17.762858393037884 16.134150707393935 14-15 47.67707573648036 18.75621560697474 15.820902119383854 17.745806537161045 16-17 47.11820656978183 20.5659276546091 16.615395495208798 15.70047028040027 18-19 45.81232016336128 20.897072668366874 17.796479069335728 15.49412809893612 20-21 46.983659740730424 20.686759395538584 17.361249232047594 14.968331631683396 22-23 47.40336082456001 19.96914971669009 17.170967143122333 15.456522315627568 24-25 46.63116022099448 20.70497237569061 17.298121546961326 15.365745856353591 26-27 47.07366876458525 20.163001202178062 17.486829078565872 15.276500954670816 28-29 47.153016260270405 19.853529393565726 18.486941840473445 14.50651250569043 30-31 46.831493251067364 20.26536078282315 18.000689478383084 14.9024564877264 32-33 46.55455128063468 20.63158825219332 17.88645554794369 14.927404919228305 34-35 47.07317504375652 20.10413167618408 17.792991885154606 15.029701394904796 36-37 47.65526934568534 19.4128670685784 18.162933366334883 14.768930219401375 38-39 47.342367427758184 19.58285820361187 18.742652063609924 14.332122305020023 40-41 46.99112032424739 19.36600264312897 18.58101985882686 15.061857173796781 42-43 46.760777519468924 19.251032007708016 18.900989136295735 15.087201336527329 44-45 46.058104124217124 19.50903650402875 19.010912755415493 15.421946616338635 46-47 46.408065663910044 19.351316946822664 18.648532772284668 15.592084616982623 48-49 46.16020402300121 19.02549845524179 19.966939080393725 14.847358441363276 50-51 46.54364640883978 18.798674033149172 19.43381215469613 15.223867403314916 52-53 45.38720270849632 19.861569672620234 19.192843346548745 15.558384272334708 54-55 45.56741036701789 19.793950215684887 18.700056573085355 15.938582844211865 56-57 45.637328783773626 19.73091593775 18.445606389420504 16.18614888905586 58-59 45.37438118811881 19.662747524752476 19.280852192362094 15.68201909476662 60-61 45.49998895052043 19.637135090937218 18.62323484563877 16.239641112903584 62-63 45.415329673729524 19.480760561139252 18.862428951532348 16.241480813598876 64-65 45.733480662983425 19.791381215469613 18.372596685082872 16.102541436464087 66-67 45.22603514727202 19.9480216399703 18.042148438881227 16.783794773876455 68-69 45.22418274075777 19.77511005816729 17.994289351319814 17.00641784975513 70-71 45.7903483880093 19.502126042025793 17.703167404814312 17.00435816515059 72-73 46.29041953220303 19.077842405812987 16.835210296483567 17.796527765500418 74-75 46.570050871354255 19.10217142453051 16.588658030469773 17.739119673645458 76 47.31896612686514 17.969910190227 16.505197652216957 18.2059260306909 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.5 4 1.0 5 0.5 6 1.0 7 2.5 8 3.0 9 3.5 10 3.5 11 10.5 12 18.0 13 22.5 14 31.5 15 30.5 16 36.5 17 48.0 18 57.0 19 83.5 20 114.5 21 128.0 22 153.0 23 231.0 24 310.0 25 336.0 26 407.5 27 540.5 28 728.5 29 855.0 30 957.0 31 1178.0 32 1514.5 33 1732.0 34 1916.5 35 2296.5 36 2661.0 37 2830.0 38 3132.0 39 3736.5 40 4301.5 41 4715.5 42 4867.0 43 4832.5 44 5036.5 45 5148.5 46 5022.0 47 4959.5 48 4578.5 49 3974.0 50 3688.0 51 3438.5 52 2911.0 53 2400.0 54 2167.0 55 2003.0 56 1625.0 57 1241.5 58 1072.0 59 936.5 60 703.5 61 549.5 62 493.0 63 444.0 64 373.0 65 333.0 66 321.0 67 327.0 68 283.0 69 218.5 70 199.5 71 201.0 72 185.0 73 180.0 74 176.5 75 162.0 76 142.0 77 139.5 78 170.5 79 184.0 80 177.5 81 164.0 82 163.0 83 169.0 84 184.0 85 209.5 86 228.5 87 237.0 88 254.0 89 287.5 90 320.0 91 381.5 92 427.0 93 479.5 94 594.0 95 757.5 96 859.0 97 1070.5 98 1832.5 99 15268.0 100 28153.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0035357865799220357 2 0.005303679869883054 3 0.0097234130947856 4 0.0 5 8.839466449805089E-4 6 8.839466449805089E-4 7 0.002651839934941527 8 8.839466449805089E-4 9 0.002651839934941527 10-11 0.0022098666124512724 12-13 0.0022098666124512724 14-15 0.005745653192373308 16-17 0.004419733224902545 18-19 0.0048617065473928 20-21 0.0030938132574317816 22-23 0.0017678932899610179 24-25 0.0035357865799220357 26-27 8.839466449805089E-4 28-29 4.4197332249025446E-4 30-31 0.0 32-33 0.0013259199674707635 34-35 0.002651839934941527 36-37 0.002651839934941527 38-39 0.0017678932899610179 40-41 0.0048617065473928 42-43 0.0 44-45 0.00397775990241229 46-47 0.006629599837353816 48-49 0.00397775990241229 50-51 0.0035357865799220357 52-53 0.0030938132574317816 54-55 8.839466449805089E-4 56-57 0.00397775990241229 58-59 0.00795551980482458 60-61 0.0013259199674707635 62-63 0.0017678932899610179 64-65 0.0035357865799220357 66-67 0.004419733224902545 68-69 0.006187626514863563 70-71 0.007071573159844071 72-73 0.002651839934941527 74-75 4.4197332249025446E-4 76 8.839466449805089E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 113129.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.13745370329447 #Duplication Level Percentage of deduplicated Percentage of total 1 83.21403385049365 41.72139769643504 2 6.875881523272215 6.894783830847969 3 3.7923131170662905 5.704107700059224 4 2.1068406205923833 4.225264963006833 5 1.2358956276445698 3.0982329906566837 6 0.7440056417489422 2.238152905090649 7 0.5077574047954866 1.782036436280706 8 0.3296897038081806 1.3223841808908414 9 0.25035260930888575 1.1296838122850905 >10 0.9291255289139634 7.101627345773409 >50 0.008815232722143865 0.2651839934941527 >100 0.0035260930888575456 0.19888799512061453 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0017630465444287728 24.31825615005878 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 27511 24.31825615005878 No Hit CTGTCTCTTATACACATCTGACGCTGCCGACGACCAAGCCCGTGTAGATC 123 0.1087254373326026 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.1785572222860628 0.0 2 0.0 0.0 0.0 0.1794411689310433 0.0 3 0.0 0.0 0.0 0.18120906222100433 0.0 4 0.0 0.0 0.0 0.23247796762987386 0.0 5 0.0 0.0 0.0 0.23866559414473743 0.0 6 0.0 0.0 0.0 0.24220138072465947 0.0 7 0.0 0.0 0.0 0.24662111394956202 0.0 8 0.0 0.0 0.0 0.24662111394956202 0.0 9 0.0 0.0 0.0 0.24838900723952304 0.0 10 0.0 0.0 0.0 0.2545766337543866 0.0 11 0.0 0.0 0.0 0.2572284736893281 0.0 12 0.0 0.0 0.0 0.2589963669792891 0.0 13 0.0 0.0 0.0 0.25988031362426967 0.0 14 0.0 0.0 0.0 0.26960372671905525 0.0 15 0.0 0.0 0.0 0.26960372671905525 0.0 16 0.0 0.0 0.0 0.26960372671905525 0.0 17 0.0 0.0 0.0 0.27048767336403573 0.0 18 0.0 0.0 0.0 0.2731395132989773 0.0 19 0.0 0.0 0.0 0.27844319316886035 0.0 20 0.0 0.0 0.0 0.28109503310380185 0.0 21 0.0 0.0 0.0 0.2819789797487824 0.0 22 0.0 0.0 0.0 0.2872826596186654 0.0 23 0.0 0.0 0.0 0.28993449955360695 0.0 24 0.0 0.0 0.0 0.30142580593835355 0.0 25 0.0 0.0 0.0 0.3023097525833341 0.0 26 0.0 0.0 0.0 0.3023097525833341 0.0 27 0.0 0.0 0.0 0.3120331656781197 0.0 28 0.0 0.0 0.0 0.3244084187078468 0.0 29 0.0 0.0 0.0 0.3500428714122816 0.0 30 0.0 0.0 0.0 0.37125759089181376 0.0 31 0.0 0.0 0.0 0.39158836372636546 0.0 32 0.0 0.0 0.0 0.4242943895906443 0.0 33 0.0 0.0 0.0 0.46583988190472825 0.0 34 0.0 0.0 0.0 0.5250643071184223 0.0 35 0.0 0.0 0.0 0.6081552917465902 0.0 36 0.0 0.0 0.0 0.7354436086237834 0.0 37 0.0 0.0 0.0 0.9095810976849438 0.0 38 0.0 0.0 0.0 1.135871438799954 0.0 39 0.0 0.0 0.0 1.3992875390041457 0.0 40 0.0 0.0 0.0 1.7457946238365052 0.0 41 0.0 0.0 0.0 2.169205066782169 0.0 42 0.0 0.0 0.0 2.6562596681664297 0.0 43 0.0 0.0 0.0 3.14331426955069 0.0 44 0.0 0.0 0.0 3.5958949517807106 0.0 45 0.0 0.0 0.0 4.013117768211511 0.0 46 0.0 0.0 0.0 4.509011836045576 0.0 47 0.0 0.0 0.0 4.941261745441045 0.0 48 0.0 0.0 0.0 5.371743761546553 0.0 49 0.0 0.0 0.0 5.810181297456886 0.0 50 0.0 0.0 0.0 6.193814141378426 0.0 51 0.0 0.0 0.0 6.673797169602842 0.0 52 0.0 0.0 0.0 7.106931025643292 0.0 53 0.0 0.0 0.0 7.546252508198605 0.0 54 0.0 0.0 0.0 7.9678950578543075 0.0 55 0.0 0.0 0.0 8.401028913894757 0.0 56 0.0 0.0 0.0 8.76963466485163 0.0 57 0.0 0.0 0.0 9.210724040696903 0.0 58 0.0 0.0 0.0 9.625295017192762 0.0 59 0.0 0.0 0.0 9.95147132919057 0.0 60 0.0 0.0 0.0 10.291790787508067 0.0 61 0.0 0.0 0.0 10.742603576448126 0.0 62 0.0 0.0 0.0 11.160710339523906 0.0 63 0.0 0.0 0.0 11.574397369374784 0.0 64 0.0 0.0 0.0 11.978360986130877 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTGCGA 15 0.0022204856 69.99381 10 CCCGTGC 15 0.0022204856 69.99381 8 TAGCCCG 30 8.335912E-6 58.328175 5 TGTGCGC 20 0.0069262185 52.518578 3 TCTAGCA 20 0.0069262185 52.518578 3 GATATTA 20 0.0069262185 52.518578 1 TAGTACT 20 0.0069262185 52.518578 49 GCGAGAA 20 0.0069383485 52.495358 13 TACTTGG 20 0.0069383485 52.495358 7 CGACGTG 20 0.0069383485 52.495358 39 CCGTGCG 20 0.0069383485 52.495358 9 TAGCCTT 20 0.0069383485 52.495358 5 CTGGTGT 20 0.0069383485 52.495358 9 CCACTTC 20 0.0069383485 52.495358 38 TGCGCGT 20 0.0069383485 52.495358 5 CCATCTA 20 0.0069383485 52.495358 28 TACGATA 20 0.0069383485 52.495358 54 TTCCACC 20 0.0069383485 52.495358 39 CCATATG 20 0.0069383485 52.495358 27 CTGTTAC 20 0.0069383485 52.495358 9 >>END_MODULE