FastQCFastQC Report
Wed 25 May 2016
SRR1780777_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780777_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences113129
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCCGT1459012.896781550265626No Hit
TCTTATACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCCG40183.5516976195316854No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCC11601.0253781081773903No Hit
CTTATACACATCTCCGAGCCCACGAGACACAAATAAATCTCGTATGCCGT4970.43932148255531295No Hit
TCTTTATACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCC4600.40661545669103416No Hit
ATACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCCGTCTT4460.394240203661307No Hit
CTTATACACATCTCCGCGCCCACGAGACCCAAATAAATCTCGTATGCCGT3370.2978900193584315No Hit
CTTATACACATCTCCGAGCCCACGAGACCCAAAAAAATCTCGTATGCCGT2630.23247796762987388No Hit
CTTATACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGACGT2160.19093247531578994No Hit
CATATACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCCGT2040.18032511557602382No Hit
CTTAAACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCCGT1980.17502143570614076No Hit
CTTATACACATCTCCGAGCCCACGAGACCAAAATAAATCTCGTATGCCGT1710.15115487629166702No Hit
CTTATACACATCTCCGAGCCCACGAGACCCAAATAAATATCGTATGCCGT1620.14319935648684246No Hit
CTTATACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCAGT1580.13966356990692042No Hit
CTTATACACATCTCCGAGCCCACGAGACCCAAATAACTCTCGTATGCCGT1570.1387796232619399No Hit
CTTATACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCCGA1570.1387796232619399No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTA1500.13259199674707636No Hit
CTATACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCCGTC1440.12728831687719327No Hit
TCTTATACACATCTCCGCGCCCACGAGACCCAAATAAATCTCGTATGCCG1340.1184488504273882No Hit
CTTATACACATCTCCGAGCCCACGAGACCCAAATAAAACTCGTATGCCGT1270.11226122391252463No Hit
CTTATACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGAATGCCGT1150.10165386417275854No Hit
TCTTAATACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCC1140.10076991752777803No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTAAGC150.002216981570.02167551
GTGTTCA150.002216981570.02167511
TGTGTTC150.002216981570.02167510
TTCGTAG150.002220875469.99071535
TTTTGTG150.002220875469.99071546
AGGGGGG18450.062.0242970
CGTCTTA308.338098E-658.32559648
ATCGTAA308.338098E-658.32559639
GACATCG252.3781552E-456.0173347
ACATCGT252.3781552E-456.0173348
TGACGTC1250.055.99257345
ACGGGGG252.383361E-455.99257370
GCCGACA252.383361E-455.99257346
ACCGTCT252.383361E-455.99257346
CCACGAC252.383361E-455.99257320
TATGACG1350.054.43722543
TTGTGTG200.006927429752.5162547
CACTGGC200.006927429752.51625412
TGTTCAG200.006927429752.51625412
AACGAGC200.006927429752.51625413
Produced by FastQC (version 0.11.5)