FastQCFastQC Report
Wed 25 May 2016
SRR1780776_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780776_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40589
Sequences flagged as poor quality0
Sequence length76
%GC93

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2812269.28478159107148No Hit
GGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG780.19217029244376554No Hit
GGGGGGGGGGGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG740.18231540565177756No Hit
GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG640.15767818867180763No Hit
GGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG550.13550469338983467No Hit
GGGGGGGGGGGGGGGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGG510.1256498065978467No Hit
GGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG510.1256498065978467No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGGGGG480.11825864150385573No Hit
GGGGGGGGGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG460.11333119810786174No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGGT207.798548E-570.0691443
CTCGGTG207.798548E-570.0691444
TTGTGGG150.002200763470.069132
ATCTCGG150.002211578469.98273542
GATCTCG150.002211578469.98273541
TGTAGAT207.846466E-569.98273537
AGATCTC150.002211578469.98273540
TCGGTGG252.3520047E-456.0553145
CGGTGGT200.006877050752.55185346
TGAGAAG200.00691074952.48705364
GGCGCTG200.00691074952.48705314
GGCTGGC200.00691074952.48705310
GTGTAGA305.819264E-446.65515536
GCTGGCG350.00124300639.9901417
GGGGCTG900.005198707319.4396514
AGGGGGG4200.00896166457.507406768
GGCGGGG8800.00111605875.566808723
Produced by FastQC (version 0.11.5)