FastQCFastQC Report
Wed 25 May 2016
SRR1780776_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780776_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40589
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCGT1397434.42804700781No Hit
TCTTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCG38889.578949961812313No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCC9112.2444504668752616No Hit
TCTTTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCC5111.258961787676464No Hit
CTTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGACGT4361.0741826603266895No Hit
CTTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCACGTATGCCGT3540.8721574810909359No Hit
CTTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTAAGCCGT3300.813028160339008No Hit
CTTATACACATCTCCGCGCCCACGAGACCTTGGGTCATCTCGTATGCCGT2830.6972332405331494No Hit
ATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCGTCTT2770.6824509103451674TruSeq Adapter, Index 7 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTAGTATGCCGT2580.6356401980832246No Hit
CTTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCGA2420.5962206509152727No Hit
CTTATACACATCTCCGAGCCCACGAGACCTTGGGTCATATCGTATGCCGT1940.47796200941141687No Hit
CTTATACACATCTCCGAGCCCACGAGACCTAGGGTCATCTCGTATGCCGT1610.3966591933775161No Hit
CTTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGAATGCCGT1520.37448569809554316No Hit
CTTATACACATCTCCGAGCCCACGAGACCATGGGTCATCTCGTATGCCGT1460.3597033679075612No Hit
TCTTATACACATCTCCGCGCCCACGAGACCTTGGGTCATCTCGTATGCCG1300.32028382073960926No Hit
CTTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCAGT1290.3178200990416123No Hit
TCTTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGACG1280.31535637734361527No Hit
CTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCGTC1240.3055014905516273No Hit
CTTATACACATCTCCGAGCCCACGAGACCTTGGGACATCTCGTATGCCGT1220.3005740471556333No Hit
CATATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCGT1160.2857917169676513No Hit
TCTTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCACGTATGCCG1160.2857917169676513No Hit
CTTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATTCCGT1150.28332799526965435No Hit
CTTAAACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCGT1120.27593683017566334No Hit
TCTTAATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCC1050.2586907782896844No Hit
CTTATACACATCTCCGAGCCCACGAGACATTGGGTCATCTCGTATGCCGT980.24144472640370546No Hit
CCTTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCG980.24144472640370546No Hit
CTTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCCT930.22912611791372048No Hit
CTTATACACATCTCCGAGCCCACGAGACCTTGGGTCAACTCGTATGCCGT830.20448890093375055No Hit
CTTTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCG800.19709773583975956No Hit
CTTTACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCGTC750.18477912734977456No Hit
TTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCGTC680.16753307546379562No Hit
TCTTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTAGTATGCCG600.14782330187981965No Hit
TATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCGTCT600.14782330187981965No Hit
TCTTATACACATCTCCGAGCCCACGAGCCCTTGGGTCATCTCGTATGCCG580.14289585848382566No Hit
TCTTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTAAGCCG580.14289585848382566No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG560.13796841508783167No Hit
TCTTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCAG560.13796841508783167No Hit
CTTATACACATCTCCGAGCCCACGAGCCCTTGGGTCATCTCGTATGCCGT550.13550469338983467No Hit
TCTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCGT550.13550469338983467No Hit
CTTATACACATCTCCGAGACCACGAGACCTTGGGTCATCTCGTATGCCGT530.1305772499938407No Hit
TCTTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATTCCG520.1281135282958437No Hit
CTTAATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCG500.12318608489984971No Hit
ATTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCGT500.12318608489984971No Hit
CTTATACACATCTCCGAGCCAACGAGACCTTGGGTCATCTCGTATGCCGT490.12072236320185273No Hit
CTTATACACATCTCCGAGCCCACGAGACCTTGGGTAATCTCGTATGCCGT470.11579491980585872No Hit
ATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCGTCAT470.11579491980585872No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCCGA252.7774968E-670.060511
ACTGCAT207.803315E-570.0604953
AATCTCC401.2914825E-1070.060499
AAATCTC401.2914825E-1070.060498
ATAAGCT309.935684E-870.0604952
AACGAGC150.0022018470.0604913
AATCAGC150.0022018470.0604951
ATCTACG353.5706762E-970.0604910
CACAACT309.935684E-870.060497
TCAACGA207.803315E-570.0604911
TCTACGA353.5706762E-970.0604911
AACTCCG309.935684E-870.0604910
TTAAGCT353.5706762E-970.0604952
TTAAGCA353.5706762E-970.0604952
TACGAGC353.5706762E-970.0604913
TACTGCA207.803315E-570.0604952
ATCATGA207.803315E-570.0604955
CTACGAG353.5706762E-970.0604912
ATCAACG207.803315E-570.0604910
AAGCTTG600.070.0604954
Produced by FastQC (version 0.11.5)