##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780776_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 40589 Sequences flagged as poor quality 0 Sequence length 76 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.882628298307424 32.0 32.0 32.0 27.0 32.0 2 30.160437556973566 32.0 32.0 32.0 27.0 32.0 3 29.667988863977925 32.0 32.0 32.0 27.0 32.0 4 30.244647564611103 32.0 32.0 32.0 27.0 32.0 5 30.316982433664293 32.0 32.0 32.0 27.0 32.0 6 33.49846017393875 37.0 32.0 37.0 27.0 37.0 7 34.005839020424254 37.0 37.0 37.0 27.0 37.0 8 31.61391017270689 37.0 32.0 37.0 13.0 37.0 9 33.705634531523316 37.0 32.0 37.0 27.0 37.0 10-11 33.52549951957427 37.0 34.5 37.0 24.5 37.0 12-13 34.03691886964449 37.0 34.5 37.0 27.0 37.0 14-15 33.63646554485206 37.0 32.0 37.0 27.0 37.0 16-17 30.222806671758356 34.5 27.0 37.0 17.5 37.0 18-19 31.82763803000813 37.0 29.5 37.0 17.5 37.0 20-21 33.66291359728005 37.0 34.5 37.0 24.5 37.0 22-23 32.289906132203306 37.0 29.5 37.0 17.5 37.0 24-25 31.31916282736702 37.0 29.5 37.0 13.0 37.0 26-27 32.19677745201902 37.0 32.0 37.0 13.0 37.0 28-29 33.08308901426494 37.0 32.0 37.0 22.0 37.0 30-31 32.676353199142625 37.0 32.0 37.0 17.5 37.0 32-33 31.401808371726332 37.0 32.0 37.0 13.0 37.0 34-35 32.410456034886295 37.0 32.0 37.0 13.0 37.0 36-37 32.961233339082014 37.0 34.5 37.0 20.0 37.0 38-39 29.055729384808693 34.5 24.5 37.0 10.5 37.0 40-41 27.875557417034173 34.5 17.5 37.0 8.0 37.0 42-43 28.82109684889995 37.0 20.0 37.0 10.5 37.0 44-45 30.864938776515807 37.0 29.5 37.0 13.0 37.0 46-47 27.96722018280815 32.0 17.5 37.0 8.0 37.0 48-49 27.799058858311366 34.5 20.0 37.0 10.5 37.0 50-51 28.34478553302619 37.0 22.0 37.0 8.0 37.0 52-53 27.68858557737318 34.5 17.5 37.0 10.5 37.0 54-55 27.330126388923105 32.0 17.5 37.0 13.0 37.0 56-57 26.13472862105497 29.5 13.0 37.0 8.0 37.0 58-59 27.29393431717953 32.0 17.5 37.0 10.5 37.0 60-61 27.947535046441153 34.5 20.0 37.0 10.5 37.0 62-63 31.676094508364336 37.0 29.5 37.0 13.0 37.0 64-65 32.69112321072212 37.0 32.0 37.0 22.0 37.0 66-67 32.75360319298332 37.0 32.0 37.0 17.5 37.0 68-69 32.25795166178028 37.0 32.0 37.0 13.0 37.0 70-71 27.20371282859888 29.5 20.0 37.0 10.5 37.0 72-73 26.676217694449235 32.0 13.0 37.0 10.5 37.0 74-75 30.50808100716943 37.0 27.0 37.0 13.0 37.0 76 32.14269876074798 37.0 32.0 37.0 13.0 37.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 11101 1 -0.9963206043355335 11101 2 -1.8464761075158727 11101 3 -1.1641939884652075 11101 4 -2.2857501433222076 11101 5 -4.686647203790194 11101 6 -1.0994909089119886 11101 7 -0.2762042999243022 11101 8 2.2893612925170252 11101 9 0.5299623968883154 11101 10-11 -1.3986648711402836 11101 12-13 -1.0615591925345882 11101 14-15 2.137431410835923 11101 16-17 -2.144342840552639 11101 18-19 1.042132195892023 11101 20-21 -1.3925221702109312 11101 22-23 -2.2323015767111016 11101 24-25 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2.1043530123181213 21611 44-45 1.9832114187081018 21611 46-47 2.3649776714226896 21611 48-49 3.906898810114864 21611 50-51 2.645310945564958 21611 52-53 0.9940787996749485 21611 54-55 4.838743495471114 21611 56-57 3.2349185047830673 21611 58-59 2.1262589127662537 21611 60-61 3.6639167661443324 21611 62-63 1.614349273343965 21611 64-65 1.5876998333260843 21611 66-67 1.2224444465505258 21611 68-69 -1.9806660278288213 21611 70-71 1.6250352969815047 21611 72-73 2.043571914523774 21611 74-75 1.5781712872072617 21611 76 -0.6718183183432487 21612 1 0.5732282678449181 21612 2 0.28134344135630585 21612 3 1.0218962370987015 21612 4 1.1052273002868134 21612 5 0.2362851270368722 21612 6 1.2106594670278596 21612 7 0.30650246699299544 21612 8 3.729210916577177 21612 9 0.13898495327929083 21612 10-11 2.0543426476567035 21612 12-13 1.641448326262406 21612 14-15 -0.15580167187084726 21612 16-17 1.3424992647105185 21612 18-19 -0.04245427027338877 21612 20-21 1.484357529037183 21612 22-23 -0.10166247896674108 21612 24-25 2.0294498502453813 21612 26-27 1.9584570724409218 21612 28-29 1.1410779623708152 21612 30-31 0.8101863319386524 21612 32-33 2.910718026663776 21612 34-35 2.5171858604381256 21612 36-37 3.0838279435981875 21612 38-39 3.2955856314844496 21612 40-41 -0.05375565520352055 21612 42-43 2.6579244408895555 21612 44-45 2.072497132993817 21612 46-47 1.061406242851259 21612 48-49 0.7104702386862947 21612 50-51 1.5203109455649582 21612 52-53 1.1726502282463755 21612 54-55 3.2851720668996833 21612 56-57 2.4670613619259285 21612 58-59 2.8048303413376807 21612 60-61 3.253202480430044 21612 62-63 1.3822064162011038 21612 64-65 2.4805569761832302 21612 66-67 0.6688730179790952 21612 68-69 1.8764768293140364 21612 70-71 1.8750352969815047 21612 72-73 1.4364290573809164 21612 74-75 3.828171287207269 21612 76 2.363895967371036 >>END_MODULE >>Per sequence quality scores pass #Quality Count 17 3.0 18 6.0 19 20.0 20 72.0 21 202.0 22 441.0 23 727.0 24 1055.0 25 1565.0 26 1955.0 27 2489.0 28 3042.0 29 3572.0 30 4199.0 31 4826.0 32 5231.0 33 5333.0 34 4135.0 35 1624.0 36 92.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 4.632253295552545 6.066280645558704 20.7441172847111 68.55734877417765 2 2.4760403064869796 6.642193697799896 68.38552317130257 22.496242824410555 3 2.648500825346769 7.807534060952475 82.11584419423981 7.428120919460937 4 1.7861982310478208 64.68254945921309 27.810490526990073 5.720761782749021 5 2.079381113109463 27.004853531745056 68.16625194018083 2.7495134149646456 6 3.626777046837657 72.16350062827999 19.29681917855471 4.9129031463276425 7 3.698046268693488 24.299687107344354 9.167508438246816 62.834758185715344 8 2.9096553253344504 71.83226982680037 4.368178570548671 20.889896277316513 9 2.821030846555632 21.020991426037252 2.941756184093821 73.2162215433133 10-11 3.159723077681145 45.78087659218015 34.77543176722757 16.283968562911134 12-13 4.057949589770123 7.278192524700026 45.29036390962624 43.373493975903614 14-15 4.451945108280569 5.257582103525586 14.198428145556678 76.09204464263716 16-17 42.69435923968612 33.47869470416497 2.683025980265346 21.143920075883564 18-19 45.427332528517574 16.04745127990342 2.817265761659563 35.707950429919435 20-21 7.228915662650602 5.463572079729963 2.598122551555917 84.70938970606352 22-23 4.72178079998029 43.53696244010003 2.6029540387055445 49.13830272121414 24-25 42.588417901622385 38.481343237616564 2.797590450497062 16.132648410263993 26-27 45.311422095200555 45.82881639893564 3.7338622252882625 5.125899280575539 28-29 7.3308942188058195 16.239821624351723 4.456927454820947 71.97235670202151 30-31 5.665328044544089 5.839020424252876 70.78272438345364 17.71292714774939 32-33 72.94117647058823 3.717893440098553 19.21034801355097 4.130582075762242 34-35 56.810958634112694 5.135627879474734 34.341816748380104 3.7115967380324717 36-37 6.452487127054128 34.70521569883466 16.18172411244426 42.66057306166696 38-39 3.951906967576624 17.678870602148418 41.65640090667193 36.71282152360303 40-41 3.83852569077448 9.555662318144302 43.27210909494069 43.33370289614053 42-43 41.69458953385237 6.405834236720214 37.04912782103085 14.850448408396572 44-45 6.761850793337933 44.5919976347689 43.782645116783286 4.863506455109885 46-47 41.18458657731349 9.722085345422292 16.57879176111166 32.51453631615256 48-49 35.508388972381674 5.896917884155806 5.0826578629678 53.51203528049472 50-51 13.923005851555281 8.360948567908839 41.71234986141053 36.003695719125346 52-53 3.344584606287573 13.605006405834235 68.5177885089189 14.532620478959299 54-55 3.1807822605481983 15.523252232830304 43.34093008931321 37.95503541730829 56-57 40.03153835729509 8.487021226792821 15.66938931391753 35.812051101994555 58-59 6.3379240680972675 15.72963118086181 63.58865702530242 14.3437877257385 60-61 39.44172066323388 38.99578703589642 18.183497992066815 3.3789943088028775 62-63 5.607430584641159 87.95856020103969 3.1929833206041045 3.241025893715046 64-65 2.3222579492675957 91.3860861637777 2.872947234849885 3.4187086521048156 66-67 2.4576229427416973 91.32502217404159 2.823494628954371 3.3938602542623437 68-69 3.9691534443677936 89.72233172366217 2.8284221937518477 3.480092638218193 70-71 21.706725142596675 71.12093922856121 3.203035492097126 3.969300136744977 72-73 81.73643105274829 10.771391263642858 3.507107837098721 3.9850698465101386 74-75 89.52153345816497 3.5564698925790874 3.1055977136099338 3.8163989356460037 76 90.35651809692759 3.1241530539335254 3.1241530539335254 3.3951757952053616 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 2.5 17 5.0 18 3.0 19 3.0 20 4.0 21 3.0 22 4.0 23 8.5 24 12.5 25 13.0 26 19.0 27 31.5 28 41.5 29 45.0 30 49.5 31 68.5 32 104.0 33 125.0 34 138.0 35 168.5 36 217.5 37 249.0 38 288.5 39 403.5 40 555.5 41 725.0 42 818.0 43 974.5 44 1455.5 45 2333.5 46 2887.0 47 4155.5 48 8777.5 49 11414.5 50 10698.0 51 5940.5 52 856.0 53 383.5 54 238.0 55 211.5 56 168.5 57 152.5 58 153.0 59 135.5 60 114.0 61 110.0 62 110.0 63 96.5 64 78.0 65 60.0 66 44.0 67 41.0 68 33.0 69 28.0 70 29.5 71 28.0 72 22.5 73 17.5 74 11.5 75 5.0 76 7.5 77 8.5 78 8.0 79 9.0 80 4.5 81 2.5 82 3.0 83 1.0 84 2.0 85 2.5 86 2.5 87 3.0 88 2.0 89 0.5 90 1.0 91 2.5 92 3.0 93 2.0 94 2.5 95 6.0 96 8.0 97 8.0 98 14.5 99 34.0 100 47.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.009854886791987977 2 0.0 3 0.0 4 0.0 5 0.0 6 0.004927443395993989 7 0.0 8 0.0 9 0.0024637216979969943 10-11 0.0 12-13 0.004927443395993989 14-15 0.0 16-17 0.0012318608489984971 18-19 0.0 20-21 0.004927443395993989 22-23 0.0012318608489984971 24-25 0.0012318608489984971 26-27 0.0024637216979969943 28-29 0.0012318608489984971 30-31 0.0 32-33 0.0036955825469954916 34-35 0.0 36-37 0.0 38-39 0.0024637216979969943 40-41 0.0012318608489984971 42-43 0.0024637216979969943 44-45 0.0024637216979969943 46-47 0.0024637216979969943 48-49 0.0 50-51 0.0036955825469954916 52-53 0.0024637216979969943 54-55 0.0036955825469954916 56-57 0.00862302594298948 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.00862302594298948 66-67 0.0024637216979969943 68-69 0.0024637216979969943 70-71 0.006159304244992486 72-73 0.0 74-75 0.0024637216979969943 76 0.004927443395993989 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 40589.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 28.396856291113355 #Duplication Level Percentage of deduplicated Percentage of total 1 88.1398577129967 25.028948729951466 2 5.708832205448551 3.2422577545640445 3 2.1863612701717856 1.8625736036857279 4 0.9456880097171612 1.0741826603266895 5 0.5552663543293423 0.7883909433590383 6 0.4598299496789866 0.7834634999630441 7 0.2949852507374631 0.5863657641232847 8 0.15616866215512754 0.3547759245115672 9 0.09543640465035572 0.24390844810170242 >10 1.0931806350858928 5.742935278030994 >50 0.14749262536873156 2.8529897262805193 >100 0.18219677251431546 9.928798442927887 >500 0.01735207357279195 3.503412254551726 >1k 0.008676036786395974 9.578949961812313 >5k 0.0 0.0 >10k+ 0.008676036786395974 34.42804700781 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCGT 13974 34.42804700781 No Hit TCTTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCG 3888 9.578949961812313 No Hit CTCTTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCC 911 2.2444504668752616 No Hit TCTTTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCC 511 1.258961787676464 No Hit CTTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGACGT 436 1.0741826603266895 No Hit CTTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCACGTATGCCGT 354 0.8721574810909359 No Hit CTTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTAAGCCGT 330 0.813028160339008 No Hit CTTATACACATCTCCGCGCCCACGAGACCTTGGGTCATCTCGTATGCCGT 283 0.6972332405331494 No Hit ATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCGTCTT 277 0.6824509103451674 TruSeq Adapter, Index 7 (95% over 21bp) CTTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTAGTATGCCGT 258 0.6356401980832246 No Hit CTTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCGA 242 0.5962206509152727 No Hit CTTATACACATCTCCGAGCCCACGAGACCTTGGGTCATATCGTATGCCGT 194 0.47796200941141687 No Hit CTTATACACATCTCCGAGCCCACGAGACCTAGGGTCATCTCGTATGCCGT 161 0.3966591933775161 No Hit CTTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGAATGCCGT 152 0.37448569809554316 No Hit CTTATACACATCTCCGAGCCCACGAGACCATGGGTCATCTCGTATGCCGT 146 0.3597033679075612 No Hit TCTTATACACATCTCCGCGCCCACGAGACCTTGGGTCATCTCGTATGCCG 130 0.32028382073960926 No Hit CTTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCAGT 129 0.3178200990416123 No Hit TCTTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGACG 128 0.31535637734361527 No Hit CTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCGTC 124 0.3055014905516273 No Hit CTTATACACATCTCCGAGCCCACGAGACCTTGGGACATCTCGTATGCCGT 122 0.3005740471556333 No Hit CATATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCGT 116 0.2857917169676513 No Hit TCTTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCACGTATGCCG 116 0.2857917169676513 No Hit CTTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATTCCGT 115 0.28332799526965435 No Hit CTTAAACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCGT 112 0.27593683017566334 No Hit TCTTAATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCC 105 0.2586907782896844 No Hit CTTATACACATCTCCGAGCCCACGAGACATTGGGTCATCTCGTATGCCGT 98 0.24144472640370546 No Hit CCTTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCG 98 0.24144472640370546 No Hit CTTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCCT 93 0.22912611791372048 No Hit CTTATACACATCTCCGAGCCCACGAGACCTTGGGTCAACTCGTATGCCGT 83 0.20448890093375055 No Hit CTTTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCG 80 0.19709773583975956 No Hit CTTTACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCGTC 75 0.18477912734977456 No Hit TTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCGTC 68 0.16753307546379562 No Hit TCTTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTAGTATGCCG 60 0.14782330187981965 No Hit TATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCGTCT 60 0.14782330187981965 No Hit TCTTATACACATCTCCGAGCCCACGAGCCCTTGGGTCATCTCGTATGCCG 58 0.14289585848382566 No Hit TCTTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTAAGCCG 58 0.14289585848382566 No Hit GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 56 0.13796841508783167 No Hit TCTTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCAG 56 0.13796841508783167 No Hit CTTATACACATCTCCGAGCCCACGAGCCCTTGGGTCATCTCGTATGCCGT 55 0.13550469338983467 No Hit TCTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCGT 55 0.13550469338983467 No Hit CTTATACACATCTCCGAGACCACGAGACCTTGGGTCATCTCGTATGCCGT 53 0.1305772499938407 No Hit TCTTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATTCCG 52 0.1281135282958437 No Hit CTTAATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCG 50 0.12318608489984971 No Hit ATTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCGT 50 0.12318608489984971 No Hit CTTATACACATCTCCGAGCCAACGAGACCTTGGGTCATCTCGTATGCCGT 49 0.12072236320185273 No Hit CTTATACACATCTCCGAGCCCACGAGACCTTGGGTAATCTCGTATGCCGT 47 0.11579491980585872 No Hit ATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCGTCAT 47 0.11579491980585872 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0024637216979969943 0.0 2 0.0 0.0 0.0 0.0024637216979969943 0.0 3 0.0 0.0 0.0 0.0024637216979969943 0.0 4 0.0 0.0 0.0 0.007391165093990982 0.0 5 0.0 0.0 0.0 0.01724605188597896 0.0 6 0.0 0.0 0.0 0.01724605188597896 0.0 7 0.0 0.0 0.0 0.01724605188597896 0.0 8 0.0 0.0 0.0 0.024637216979969942 0.0 9 0.0 0.0 0.0 0.024637216979969942 0.0 10 0.0 0.0 0.0 0.024637216979969942 0.0 11 0.0 0.0 0.0 0.024637216979969942 0.0 12 0.0 0.0 0.0 0.027100938677966938 0.0 13 0.0 0.0 0.0 0.027100938677966938 0.0 14 0.0 0.0 0.0 0.027100938677966938 0.0 15 0.0 0.0 0.0 0.02956466037596393 0.0 16 0.0 0.0 0.0 0.0443469905639459 0.0 17 0.0 0.0 0.0 0.0443469905639459 0.0 18 0.0 0.0 0.0 0.05173815565793688 0.0 19 0.0 0.0 0.0 0.06405676414792186 0.0 20 0.0 0.0 0.0 0.07391165093990983 0.0 21 0.0 0.0 0.0 0.08130281603390081 0.0 22 0.0 0.0 0.0 0.0886939811278918 0.0 23 0.0 0.0 0.0 0.0886939811278918 0.0 24 0.0 0.0 0.0 0.0886939811278918 0.0 25 0.0 0.0 0.0 0.09608514622188277 0.0 26 0.0 0.0 0.0 0.09608514622188277 0.0 27 0.0 0.0 0.0 0.11825864150385572 0.0 28 0.0 0.0 0.0 0.12072236320185271 0.0 29 0.0 0.0 0.0 0.13304097169183768 0.0 30 0.0 0.0 0.0 0.1724605188597896 0.0 31 0.0 0.0 0.0 0.18970657074576855 0.0 32 0.0 0.0 0.0 0.2094163443297445 0.0 33 0.0 0.0 0.0 0.2537633348936904 0.0 34 0.0 0.0 0.0 0.27347310847766637 0.0 35 0.0 0.0 0.0 0.3079652122496243 0.0 36 0.0 0.0 0.0 0.36216708960555816 0.0 37 0.0 0.0 0.0 0.45332479243144697 0.0 38 0.0 0.0 0.0 0.5543373820493237 0.0 39 0.0 0.0 0.0 0.6602774150631945 0.0 40 0.0 0.0 0.0 0.7785360565670502 0.0 41 0.0 0.0 0.0 0.9386779669368548 0.0 42 0.0 0.0 0.0 1.0618640518367046 0.0 43 0.0 0.0 0.0 1.1776589716425632 0.0 44 0.0 0.0 0.0 1.325482273522383 0.0 45 0.0 0.0 0.0 1.4930153489861786 0.0 46 0.0 0.0 0.0 1.6605484244499742 0.0 47 0.0 0.0 0.0 1.8009805612358027 0.0 48 0.0 0.0 0.0 1.9044568725516766 0.0 49 0.0 0.0 0.0 2.0448890093375054 0.0 50 0.0 0.0 0.0 2.128655547069403 0.0 51 0.0 0.0 0.0 2.274015127251226 0.0 52 0.0 0.0 0.0 2.3750277168691025 0.0 53 0.0 0.0 0.0 2.4957500800709553 0.0 54 0.0 0.0 0.0 2.559806844218877 0.0 55 0.0 0.0 0.0 2.6583557121387567 0.0 56 0.0 0.0 0.0 2.719948754588682 0.0 57 0.0 0.0 0.0 2.8086427357165733 0.0 58 0.0 0.0 0.0 2.919510212126438 0.0 59 0.0 0.0 0.0 2.998349306462342 0.0 60 0.0 0.0 0.0 3.099361896080219 0.0 61 0.0 0.0 0.0 3.1880558772081105 0.0 62 0.0 0.0 0.0 3.2644312498460173 0.0 63 0.0 0.0 0.0 3.343270344181921 0.0 64 0.0 0.0 0.0 3.395008499839858 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACTCCGA 25 2.7774968E-6 70.0605 11 ACTGCAT 20 7.803315E-5 70.06049 53 AATCTCC 40 1.2914825E-10 70.06049 9 AAATCTC 40 1.2914825E-10 70.06049 8 ATAAGCT 30 9.935684E-8 70.06049 52 AACGAGC 15 0.00220184 70.06049 13 AATCAGC 15 0.00220184 70.06049 51 ATCTACG 35 3.5706762E-9 70.06049 10 CACAACT 30 9.935684E-8 70.06049 7 TCAACGA 20 7.803315E-5 70.06049 11 TCTACGA 35 3.5706762E-9 70.06049 11 AACTCCG 30 9.935684E-8 70.06049 10 TTAAGCT 35 3.5706762E-9 70.06049 52 TTAAGCA 35 3.5706762E-9 70.06049 52 TACGAGC 35 3.5706762E-9 70.06049 13 TACTGCA 20 7.803315E-5 70.06049 52 ATCATGA 20 7.803315E-5 70.06049 55 CTACGAG 35 3.5706762E-9 70.06049 12 ATCAACG 20 7.803315E-5 70.06049 10 AAGCTTG 60 0.0 70.06049 54 >>END_MODULE